Hexadecanoic acid, 2-hexyl-3-hydroxy-5-(phenylmethoxy)-, (2S,3S,5R)-, compd. with (S)-.alpha.-methylbenzenemethanamine (1:1)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Scientific and detailed study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

(2S,3S,5R)-2-hexyl-3-hydroxy-5-(phenylmethoxy)-Hexadecanoic acid compd. with (S)-α-methylbenzenemethanamine (1:1)

EC Number:

604-018-5

Cas Number:

137433-01-3

Molecular formula:

C29 H50 O4 . C8 H11 N

IUPAC Name:

(2S,3S,5R)-2-hexyl-3-hydroxy-5-(phenylmethoxy)-Hexadecanoic acid compd. with (S)-α-methylbenzenemethanamine (1:1)

Method

Target gene:

his, tryp

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not applicable

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A

Metabolic activation:

with and without

Metabolic activation system:

rat liver S9 (Arcoclor 1254 induced)

Test concentrations with justification for top dose:

0.033, 0.1, 0.33, 1, 3.33 and 5 mg

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: a minimum of three non-toxic doses were required to evaluate assay data.

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Test results 2

Species / strain:

E. coli WP2 uvr A

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with and without metabolic activation

The results of the Salmonella-Escherichia coZi/Mammalian-Microsome Reverse Mutation Assay
with a Confirmatory Assay indicate that under the conditions of this study, Roche Colorado
Corporation’s test article, Ro 40-8168/002 (PE Salt), did not cause a positive increase in the mean
number of revertants per plate with any of the tester strains either in the presence or absence of
microsomal enzymes prepared from AroclorTM-induced rat liver (S9).

Executive summary:

Under the conditions of the study, Ro 40-8168/002 did not cause a positive increase in the number of

revertants per plate in any of the tester strains either in the presence or absence of metabolic activation.

Based on these results, it can be concluded that neither Ro 40-8168/002 THL-PE Salt per se, nor any of the formed

metabolites is mutagenic in the Ames test under the described experimental conditions.