7-amino-4-hydroxynaphthalene-2-sulphonic acid

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from J-CHECK and EnviChem authoritative databases

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Principles of method if other than guideline:

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I).

GLP compliance:

not specified

Oxygen conditions:

not specified

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Concentration of sludge: 30 mg/l

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

other: BOD and UV-Vis

Details on study design:

Standard type

Key result

Parameter:

other: BOD

Value:

6.3

Sampling time:

14 d

Remarks on result:

other: Other details not known

Key result

Parameter:

% degradation (TOC removal)

Value:

8.4

Sampling time:

14 d

Remarks on result:

other: Other details not known

Key result

Parameter:

other: UV-Vis

Value:

0

Sampling time:

14 d

Remarks on result:

other: Other details not known

Details on results:

Test substance undergoes 6.3, 8.4 and 0% degradation by BOD, TOC removal and UV-Vis parameter in 14 days.

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The percentage degradation of test chemical was determined to be 6.3, 8.4 and 0% by BOD, TOC removal and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemiical is considered to be not readily biodegradable in water.

Executive summary:

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 6.3, 8.4 and 0% by BOD, TOC removal and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 14 days for evaluating the percentage biodegradability of test chemical (authoritative databases, 2018). The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 6.3, 8.4 and 0% by BOD, TOC removal and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Various experimental key and supporting studies of the test chemical were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from authoritative databases (2018),biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of test chemical. The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test. Activated sludge was used as a test inoculums for the study. Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test chemical was determined to be 6.3, 8.4 and 0% by BOD, TOC removal and UV-Vis parameter in 14 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

Another biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical (GREIM H. et. al., 1994). The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test).The percentage degradation of test chemical was determined to be 0% by BOD parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

In a supporting study, biodegradation experiment was conducted for 30 days for evaluating the percentage biodegradability of test chemical (handbook, 2008). The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test). Initial test substance conc. used in the study was in the range 3-30 mg/l. The percentage degradation of test chemical was determined to be 0% by ThOD parameter in 30 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

 

On the basis of above results for test chemical, it can be concluded that the test chemical can be considered to be not readily biodegradable in nature.