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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Directive 79/831/EEC, Annex V, C
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
EC Number:
406-640-0
EC Name:
A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide
Cas Number:
136920-07-5
Molecular formula:
C58H114N4O6 - C154H308N6O4
IUPAC Name:
Reaction mass of 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C12-C18)alkylacetamide and {[2-(Carboxymethyl-di(C12-C18)alkylcarbamoylmethyl-amino)-ethyl]-di(C12-C18)alkylcarbamoylmethyl-amino}-acetic acid
Constituent 2
Reference substance name:
Keroflux ES 3241
IUPAC Name:
Keroflux ES 3241
Details on test material:
Test Substance No.: 90/763
Batch No.: Technikumspartie 1, T 75492/ST 1414/90

Water solubility: < 1 mg/L

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The concentration control analysis was conducted by extraction of the test substance with Freon (1,1,2-trifluorotrichloroethane) from the water samples.

Test solutions

Vehicle:
no
Details on test solutions:
The test was performed with an aqueous extract (eluate) prepared with 100 mg/L. The test substance was reduced to small pieces by means of a mortar, an appropriate amount weighed out and suspended in test medium by stirring for 17 hours. Then, the suspension was filtered and diluted with test medium to the desired test concentrations between 0.31 and 80 mg/L (nominal).

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
The test strain of Scenedesmus subspicatus CHODAT SAG 86.81 is obtained at regular intervais from SAG (Collection of algal cultures in Göttingen) and is kept in liquid culture in the "Labor für Okotoxikologie" at BASF AG Ludwigshafen.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +/- 2 °C
Nominal and measured concentrations:
Test concentrations (nominal): 0.31, 0.63, 1.25, 2.50, 5.00, 10.00, 20.00, 40.00, 80.00 mg/L
Details on test conditions:
- Test vessels: Erlenmeyer flasks (nominal volume 250ml) plugged with gas permeable siliconesponge caps
- Test volume: 100 ml
- Inoculation density: 10000 cells/ml
- Number of replicates/concentration: 3
- Number of replicates/control: 3
- Illumination: Artificial light, OSRAM L25 universal white, permanent illumination

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
> 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Effect on the development of biomass:
-------------------------------------
EC10 > 80 mg/L
EC50 > 80 mg/L
EC90 > 80 mg/L

Applicant's summary and conclusion