A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Directive 79/831/EEC, Annex V

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

EC Number:

406-640-0

EC Name:

A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

Cas Number:

136920-07-5

Molecular formula:

C58H114N4O6 - C154H308N6O4

IUPAC Name:

Reaction mass of 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C12-C18)alkylacetamide and {[2-(Carboxymethyl-di(C12-C18)alkylcarbamoylmethyl-amino)-ethyl]-di(C12-C18)alkylcarbamoylmethyl-amino}-acetic acid

Constituent 2

Reference substance name:

Keroflux ES 3241

IUPAC Name:

Keroflux ES 3241

Details on test material:

Test Substance No.: 90/763
Batch No.: T 75492/ST 1414/90

Water solubility: < 0.6 mg/L (Analytical Report 90.25.1, 24 Jun 1991)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

For the concentration control analysis, samples from the controls and the highest test concentration were taken at 0 and 48 hours and analyzed by DOC determination.

Test solutions

Vehicle:

yes

Details on test solutions:

Cremophor RH 40, 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: Institut National de Recherche Chimique Appliquée, France
- Age of the animals at the start of the test: 2-24 hours
- Age of stock animals: 2-4 weeks

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Total hardness 2.63 mmol/L

Test temperature:

20.1-20.8 °C

pH:

7.9-8.0

Dissolved oxygen:

7.4-8.6 mg/L

Nominal and measured concentrations:

The test substance was tested in the following range of concentrations: 100 to 12.5 mg/L. In parallel, a control and a solvent control were run.

Details on test conditions:

- Test vessels: Tubes (glass) with flat bottom
- Volume of test vessels: Nominal 20 ml
- Test volume: 10 ml
- Number of animals/vessel: 5
- Total number of animals/conc.: 20
- Number of parallels/conc.: 4
- Number of parallels/control: 4
- Light: Artificial light, day/night-rhythm 16/8 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

24h EC0 > 100 mg/L

Any other information on results incl. tables

Summary of the concentration control analysis:
----------------------------------------------

 Time     Stocked with         Minimum           Maximum
 [h]       daphnids             [mg/L]            [mg/L]
-----------------------------------------------------------
  0           no                 42.8              42.8
 48           no                 50.0              50.0
 48           yes                41.5              41.5
-----------------------------------------------------------

The values given are the minimum and maximum concentrations of test  substance in the test solutions at various times of the study. 
The values are given in mg/L DOC and corrected for DOC of the solvent control.