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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug 16, 2000 - Sep 13, 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 301F "Ready Biodegradability: Manometric Respirometry Test".

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Principles of method if other than guideline:
None
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name: CCP-V-1

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, adapted
Details on inoculum:
The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted.
The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot
of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre
were mixed with test water and then aerated until use.
Duration of test (contact time):
>= 28 d
Initial test substance concentration
Initial conc.:
104 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
according to guideline
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
Procedure Control: 24.9 mg Aniline and activated sludge at a concentration of 30 mg suspended solids per litre were filled up with test water to a volume of 244 mL. The procedure control was also used for other projects which ran in parallel. The reference item Aniline was sufficiently degraded to 77 % after 14 days, and to 92 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
% Degradation
Parameter:
% degradation (O2 consumption)
Value:
ca. 2
Sampling time:
28 d
Details on results:
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period degradation rates of 2 % and 3 % were found. CCP-V-1 was found to be not ready biodegradable under the test conditions within 28 days.
The reference item Aniline was sufficiently degraded to 77 % after 14 days, and to 92 % after 28 days of incubation.
The biodegradation in the toxicity control containing both, the test item and the reference item Aniline was determined to be 29 % within 14 days and 32 % biodegradation was determined after 28 days of incubation.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not readily biodegradable
Conclusions:
CCP-V-1 was found to be not ready biodegradable under the test conditions within 28 days.
Executive summary:

Purpose

The test item CCP-V-1 was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item Aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.

Result

Biodegradation of CCP-V-1

After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28-day exposure period degradation rates of 2 % and 3 % were found.

Biodegradation of Aniline

The reference item Aniline was sufficiently degraded to 77 % after 14 days, and to 92 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.

Biodegradation in the Toxicity Control

In the toxicity control containing both, the test item and the reference item Aniline 29 % biodegradation was noted within 14 days and 32 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge micro organisms.

Conclusion

CCP-V-1 was found to be not ready biodegradable under the test conditions within 28 days.