6H-Furo[2',3':4,5]oxazolo[3,2-a]pyrimidin-6-one, 2,3,3a,9a-tetrahydro-3-hydroxy-2-(hydroxymethyl)-7-methyl-, (2S,3S,3aR,9aS)-

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 May - 15 October 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

35°C +/- 1.0°C

Details on study design: HPLC method:

EQUIPMENT
Instrument: Alliance Separation Module 2695
Detector: Dual λ Absorbance Detector 2487 (Waters)
Column: 150 mm x 4.6 mm i.d. Hypersil BDS-CN; dp = 5 µm (Thermo, San Jose, CA, USA)
Column temperature: 35°C +/- 1.0°C
Mobile phase: 55/45 (v/v) methanol/water
Flow : 1.0 ml/min
Injection volume: 10 µl
UV detection: 210 nm


REFERENCE SUBSTANCES
- Identity: A stock solution of phenol in methanol at a concentration of 1.23 g/l was used. The phenol reference substance solution was prepared by adding 50 µl of the stock solution to a 5 ml volumetric flask. The flask was filled up to the mark with mobile phase.


TEST SOLUTIONS:
A 884 mg/l stock solution of the test substance was prepared in methanol. In order to dissolve the test substance completely the stock solution was ultrasonicated for 10 minutes.
The test solution was prepared by adding 50 µl of the stock solution to a 5 ml volumetric flask. The flask was filled up to the mark with mobile phase. The final concentration of the test substance solution was 8.84 mg/l.


REPETITIONS
- Number of determinations: The reference substance and test substance solutions were injected in duplicate. Blank solutions were analysed by single injection.

Analytical monitoring:

no

Details on matrix:

The dual composition of a cyanopropyl stationary phase, having polar and non-polar sites allows for interaction of polar and non-polar groups of a molecule in a similar way as is the case for organic matter in soil or sewage sludge matrices. This enables the relationship between the retention time on the column and the K,0 on organic matter to be established.

Details on test conditions:

TEST CONDITIONS
- pH: neutral

Key result

Sample No.:

#1

Type:

Koc

Value:

< 20.9 dimensionless

pH:

7

Temp.:

35 °C

Matrix:

The final concentration of the test substance solution was 8.84 mg/I.

Key result

Sample No.:

#1

Type:

log Koc

Value:

< 1.32 dimensionless

pH:

7

Temp.:

35 °C

Matrix:

The final concentration of the test substance solution was 8.84 mg/I.

Details on results (HPLC method):

- Retention times of reference substances used for calibration: retention time of phenol (log Koc = 1.32) was 2.30 minutes

- Average retention data for test substance: one peak with a retention time of 1.97 minutes was observed

Transformation products:

not measured

Validity criteria fulfilled:

yes

Conclusions:

The Koc value of the test substance is: < 20.9
The log Koc value of the test substance is: < 1.32
All measurements were performed in neutral pH.

Description of key information

The Koc value of the test substance is: < 20.9

The log Koc value of the test substance is: < 1.32

All measurements were performed in neutral pH.