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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
5. June to 3. July, 1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: domestic sewage treatment plant Frankfurt Sindlingen, Germany
- Concentration of sludge: 10 ml/L
Duration of test (contact time):
28 d
Initial conc.:
60.9 mg/L
Based on:
test mat.
Remarks:
39.65 mg as carbon
Initial conc.:
32.6 mg/L
Based on:
test mat.
Remarks:
21.22 mg as carbon
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
The test substance was directly weighed into the test vessels (5L glass vessels)
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
< 20
Sampling time:
28 d

The degradation of the reference substance caused CO2-formation amounting to more than 60 % of the theoretical maximum within 10 days.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The test substance was found to be not readily biodegradable.
Executive summary:

The testing according to the modified Sturm test (OECD Guideline 301 B) revealed that the test item was not readily biodegradable. Within a period of 28 days the CO2 formation stayed below 20 % of the theoretical maximum.

Description of key information

The testing according to the modified Sturm test (OECD Guideline 301 B) revealed that the test item was not readily biodegradable. Within a period of 28 days the CO2 formation stayed below 20 % of the theoretical maximum.

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:
freshwater

Additional information