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EC number: 605-404-6
CAS number: 16535-55-0
- based on statistical significance,#- based on biological
The objectiveofthe study was to evaluate CH02672 for its
ability to generate toxic effects in Raphidocelis subcapitata during
an exposure period of 72 hours and, if possible, to determine the NOEC,
EC10, EC20and EC50for both inhibition
of growth rate and inhibition of yield.
The study procedures described in this report were based on the
OECD guideline No. 201, 2006; Annex 5 corrected 28 July 2011. In
addition, procedures were based on the test methods described in the
OECD series on testing and assessment number 23,2019.
The batch of CH02672 tested was an off-white crystalline powder with a
purity of >99.9% and not completely soluble in test medium at the
loading rate initially prepared.
A Saturated Solution (SS) was prepared at a loading rate of 100
mg/L and used as the highest concentration. Lower concentrations were
prepared by diluting the highest concentration in test medium.
A final test was performed based on the results of a preceding combined
limit/range-finding test.Six replicates of exponentially growing
algal cultures were exposed to an untreated control, whereas three
replicates per group were exposed to solutions containing 1.0, 3.2, 10,
32 and 100% of the SS prepared at a loading rate of 100 mg/L. The
initial algal cell density was 104cells/mL. The total
exposure period was 72 hours and samples for analytical confirmation of
exposure concentrations were taken at the start, after 24 and 72 hours
Samples taken from all test concentrations and the control were
analysed. The measured concentrationsat the start of the testwere
0.90, 3.0, 8.8, 28 and 92 mg/L, respectively. During the exposure
period, the concentrations decreased to 41 – 66% of initial at the end
of the test.Based on these results, the average exposure
concentrations were calculated and used to express effect parameters.
Inhibition of growth rates and yield increased with increasing
concentration of CH02672 from 2.1 mg/L upwards resulting in 100%
inhibition at the highest concentration tested (74 mg/L)). Statistically
significant inhibition of growth rates and yield was found at test
concentrations of 2.1 mg/L and higher. However, growth rate inhibition
was considered to be biologically not relevant at 2.1 mg/L because the
observed inhibition was below 10%. Biologically relevant inhibition of
growth rate was observed at concentrations of 6.7 mg/L and higher.
The study met the acceptability criteria prescribed by the study
plan and was considered valid.
The effect parameters obtained in this study are summarized in the
– confidence limit,*
- based on statistical significance,#-
based on biological relevance.
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