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EC number: 605-404-6
CAS number: 16535-55-0
objective of the study was to evaluate
test item CH02672 for its ready
biodegradability in an aerobic aqueous
medium with microbial activity introduced
by inoculation with activated sludge.
described in this report were in
compliance with OECD guideline No. 301 B,
1992. In addition, procedures were
designed to meet test methods of ISO
standard 10634, 1995.
was an off-white crystalline powder with a
purity of >99.9% (HPLC). The test item was
tested in duplicate at a target
concentration of 23 mg/L, corresponding to
12 mg TOC/L. Organic carbon content was
based on the molecular formula.
of CH02672 was calculated to be 1.93 mg CO2/mg.
study consisted of six bottles:
inoculum blanks (no test item),
test bottles (CH02672),
procedure control (sodium acetate) and
CH02672 was not sufficiently soluble to
allow preparation of an aqueous solution
at a concentration of 1 g/L, weighed
amounts were added to 2 litre test bottles
containing medium with microbial organisms
and mineral components. To this end, 10 mL
of Milli-RO water was added to each
weighing bottle containing test item.
After vigorous mixing (vortex) the
resulting suspension was added
quantitatively to the test medium. Test
solutions were continuously stirred during
the test to ensure optimal contact between
test item and test organisms. Test
duration was 28 days for inoculum blank
and test item (last CO2measurement
on day 29) and 14 days for procedure and
toxicity control (last CO2
on day 15). Relative
biodegradation values calculated from
measurements performed during the test
period (28 days) revealed 84% and 96%
biodegradation of CH02672, for vessel A
and B, respectively (based on ThCO2).
However, biodegradation did not exceed 60%
within 10 days of achieving 10%.
the toxicity control, CH02672 was found
not to inhibit microbial activity. Since
all criteria for acceptability of the test
were met, this study was considered to be
conclusion, CH02672 was not readily
biodegradable under the conditions of the
modified Sturm test presently performed.
Since results of the present test indicate
that the pass level criterion was
fulfilled but the 10-day window criterion
was not fulfilled, the present test can be
used to indicate inherent biodegradability.
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