Nickel bis(dibutyldithiocarbamate)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

8 Jul 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1981

GLP compliance:

no

Specific details on test material used for the study:

- Name of test item (as cited in study report): Nickeldibutyldithiocarbamate (NiDBC)
- Source: Akzo Chemicals Dordrecht
- Purity: 97%

Analytical monitoring:

not specified

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution in acetone was made, from which a second stock solution in dilution water was prepared.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

- Common name: Guppy
- Source: Inhouse (Akzo Research Laboratories Arnhem, dpt. CRL)
- Length at study initiation: ≤ 2 cm

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21 - 25 °C

Details on test conditions:

TEST SYSTEM
The test organisms were bred in the Akzo Research Laboratories Arnhem, dpt. CRL. The fish were kept and tested at 23 ± 2 °C. Young fish, ≤ 2 cm, were used in the test.Test containers were 2 L glass aquaria filled with 1 L test medium. The test medium was refreshed after 48 hours. Observations on survival were made daily. Measurements of oxygen concentration, pH and temperature were carried out to confirm that the test conditions were normal. The loading in the glass vessels was approximately 0.6 g biomass/L. The number of organisms per vessel was 10.

TEST MEDIUM
The dilution water was a synthetic medium, Dutch Standard Water, containing 100 mg NaHCO3/L, 20 mg KHCO3/L, 200 mg CaCl2.2H2O/L and 180 mg MgSO4.7H2O/L. The medium was aerated before use. The medium was renewed after two or three days.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: saturated concentration

Reported statistics and error estimates:

The 96-h LC50 was determined with a programme of RIZA (Griffioen, not published) based on the LC50 model of Kooijman (1981). This model includes the data from each observation time in its calculation of the LC50. Therefore it enables calculation of 96-h LC50 values even in cases where only one or no concentration with partial mortality occurred. The model also corrects for control mortality.

Validity criteria fulfilled:

not specified

Description of key information

The 96-h LC50 value is >16 mg/L in guppies (Poecilia reticulata).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

16 mg/L

Additional information

The short-term toxicity to freshwater fish was determined in a non-GLP study similar to OECD TG 203. In this study, groups of 10 guppies (Poecilia reticulata) were exposed for 96 hours to at least a saturated concentration of 16 mg/L. The study was semi-static with a 48-hour renewal of test media. No analytical confirmation was performed. Observations on survival were made daily. Measurements of oxygen concentration, pH and temperature were carried out to confirm that the test conditions were normal. The loading in the glass vessels was approximately 0.6 g biomass/L. After 96 hours, the 96-hour LC50 was determined to be >16 mg/L.