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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Study currently in draft
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
NA (study was performed according to guidelines).
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
NA
Post exposure observation period:
NA
Salinity:
NA
Nominal and measured concentrations:
Nominal test concentrations: 0.10, 0.32, 1.0, 3.2, 10 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Effects of test item on Daphnia magna over a 48-hour exposure period 

Nominal Concentration (mg/l)

Percentage Immobilisation at

24 Hours (%)

Percentage Immobilisation at

48 Hours (%)

Control

5

5

0.10

10

10

0.32

5

5

1.0

0

0

3.2

0

25

10

85

100

Chemical analysis of test preparations 

Nominal Concentration (mg/l)

0 Hours

24 Hours (Old)

24 Hours (Fresh)

48 Hours (Old)

Measured Concentration (mg/l)

% of Nominal

Measured Concentration (mg/l)

% of Nominal

Measured Concentration (mg/l)

% of Nominal

Measured Concentration (mg/l)

% of Nominal

Control

<LOQ

-

<LOQ

-

<LOQ

-

<LOQ

-

0.10

0.157

157

0.103

103

0.182

182

0.0775

78

0.32

0.569

178

0.372

116

0.784* / 0.326**

245* / 102**

0.502

157

1.0

1.63* / 0.935**

163* / 94**

1.23

123

1.77* / 0.967**

177* / 97**

1.05

105

3.2

4.04

126

3.15

98

4.36

136

3.23

101

10

13.3

133

9.70

97

16.2* / 10.5**

162* / 105**

11.0

110

10 (RO Water)

12.8

128

12.2

122

13.2

132

14.1

141

 P. Rec 0.104          0.0997  96    
 P. Rec 0.104          0.102  98    

*outside calibration range as actual concentration much higher than nominal concentrations

**duplicate samples stored prior to analysis

Conclusions:
Exposure of Daphnia magna to the test item over a 48-hour period resulted in an EC50 value of 3.7 mg/L based on immobilisation.
Executive summary:

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to the aquatic invertebrate Daphnia magna was assessed at a GLP accredited laboratory according to the OECD Guideline 202 ("Daphnia sp. Acute Immobilisation Test").

Exposure of test organisms to the test item over a 48-hour period resulted in an EC50 value of 3.7 mg/L based on immobilisation.

Description of key information

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to the aquatic invertebrate Daphnia magna was assessed at a GLP accredited laboratory according to the OECD Guideline 202 ("Daphnia sp. Acute Immobilisation Test").

Exposure of test organisms to the test item over a 48-hour period resulted in an EC50 value of 3.7 mg/L based on immobilisation.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
3.7 mg/L

Additional information