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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Study currently in draft
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
NA (study was performed according to guidelines).
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Remarks on exposure duration:
NA
Post exposure observation period:
NA
Salinity:
NA
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.

Chemical analysis of test preparations

Nominal Concentration (mg/L)

0 hours (Fresh)

24 hours (Old)

72 hours (Fresh)

96 hours (Old)

Measured Concentration (mg/L)

% of nominal

Measured Concentration (mg/L)

% of nominal

Measured Concentration (mg/L)

% of nominal

Measured Concentration (mg/L)

% of nominal

Control

<LOQ

-

<LOQ

-

<LOQ

-

<LOQ

-

3.2

3.04

95

2.68

84

2.91

91

5.11

160

100 – RO water

94.2

94

-

-

85.5

86

6.56

7

P. Rec (0.102)

0.0960

94

0.120

109

0.120

109

0.120

109

P. Rec (0.102)

0.0944

93

0.122

111

0.122

111

0.122

111

Conclusions:
No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.
Executive summary:

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to fish was assessed at a GLP accredited laboratory according to the OECD Guideline 203 ("Fish, Acute Toxicity Test").

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.

Description of key information

The acute toxicity of the test item, Reaction mass of disodium N,N'-[(2-hydroxy-5-nonylphen-1,3-ylene)bis(methylene)]bis[N-methylaminoacetate] and sodium N-[(2-hydroxy-5-nonylphenyl)methyl]-N-methylaminoacetate, to fish was assessed at a GLP accredited laboratory according to the OECD Guideline 203 ("Fish, Acute Toxicity Test").

No mortality or sub-lethal effects of exposure were observed in fish exposed to either the control or nominal limit concentration of 3.2 mg/L over the 96-hour exposure period, thus the 96-hour LC50 value is >3.2 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
3.2 mg/L

Additional information