Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with detailed documentation (read-across: study conducted with a similar substance)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Fraunhofer IME

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solution was prepared in dilution water by weighing 200 mg of the test item, transferring it into a glass bottle and dissolving it in 2 L of dilution water. The solution was stirred for 2 h at about 300 rpm.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene
- Age at study initiation: <24 h
- Method of breeding: Adult Daphnia, at least 3 weeks old, were separated from the stock population by sieving. Batches of 30 - 50 animals were held at room temperature in ca. 1.8 L of dilution water for one week. Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. Thirty millilitres of this suspension was given to 1 L of medium. The water was changed once per week.
- Feeding during test: no
- Food type: algal suspension (Desmodesmus subspicatus) and LiquizellR (HOBBY)

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
20.3°C
pH:
7.85 – 8.40
Dissolved oxygen:
5.1 – 9.2 mg/L
Nominal and measured concentrations:
100.0, 50.0, 25.0, 12.5, 6.25 mg/L (nominal concentrations)
Details on test conditions:
TEST SYSTEM
- Test vessel: 60 mL glass beakers
- Aeration: not during test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water (filtration with activated charcoal, passage through a lime-stone column and aeration)

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle 16/8 hours
- Light intensity: 499 – 509 lux

EFFECT PARAMETERS MEASURED :
Immobilisation and sublethal effects, determined after 24 and 48 h
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Results with reference substance (positive control):
24h-EC50 = 1.03 mg/L

Any other information on results incl. tables

No statistically significant effects on immobilisation of Daphnia magna were observed up to the highest test concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes