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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with detailed documentation (read-across: study conducted with a similar substance)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Fraunhofer IME

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
- Name of test material (as cited in study report): Acylglycinate GCK-11-T
- Physical state: White to pale yellow powder, a slightly characteristic odor
- Lot/batch No.: 10825
- Expiration date of the lot/batch: August 25, 2014
- Storage condition of test material: Dark and cool place, protected from light
- ThoD: 2.060 mg O2/mg

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany. Mainly fed with municipal wastewater
- Method of cultivation: samples were not washed with mineral medium after the arrival at the laboratory but kept aerobic until use
- Initial cell/biomass concentration: 29.6 mg dry mass/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
100 mg/L
Based on:
test mat.
Initial conc.:
206 mg/L
Based on:
ThOD/L
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 22 °C ± 1°C
- pH: 7.2 - 7.8
- pH adjusted: yes

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (sterilized with HgCl2)
- Toxicity control: yes
Reference substance
Reference substance:
benzoic acid, sodium salt
Remarks:
100 mg/L

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
85.9
Sampling time:
14 d
Remarks on result:
other: mean of 2 determinations
Parameter:
% degradation (O2 consumption)
Value:
93.7
Sampling time:
28 d
Remarks on result:
other: mean of 2 determinations

Any other information on results incl. tables

The biodegradation within the 10-day-window was 82.5 %. The 10-day-window starts at day 1.

No significant abiotic degradation of Acylglycinate GCK-11-T was noticeable after 28 days of incubation.

The biodegradation of the item mixture in the toxicity control was found to be 87.9 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item can be identified as non-toxic in a ready biodegradability test.

The reference item sodium benzoate was degraded to 88.6 % within the first 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable