2,3,5-trimethylhydroquinone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19.06.2009 - 02.07.2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,3,5-trimethylhydroquinone

EC Number:

211-838-3

EC Name:

2,3,5-trimethylhydroquinone

Cas Number:

700-13-0

Molecular formula:

C9H12O2

IUPAC Name:

2,3,5-trimethylhydroquinone

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material (as cited in study report): 2,3,5-Trimethylhydrochinone
- Physical state: solid
- Analytical purity: 99.64 area-%
- Lot/batch No.: 48/130509

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared for each test concentration separately. The test item was added to the test medium and the mixture was stirred (magnetic stir plate) for about 4 hours at approximately 20°C.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used in this study was originally obtained from the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of the BASF SE, Ludwigshafen, Germany.
- Age at study initiation (mean and range, SD): < 24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

20 ± 1 °C

pH:

7,5 - 8,5

Conductivity:

550 - 650 µS/cm

Nominal and measured concentrations:

Nominal test concentrations:
0 (control), 0.46, 1.0, 2.2, 4.6, 10 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers (nominal volume 100 mL)
- Fill volume: 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/mL


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water prepared generally according to OECD 202 for M4 medium
- Ca/mg ratio: about 4 : 1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 48 hour EC50: 1 - 10 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate (Kaliumdichromat, K2Cr2O7)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test item is not completely stable in test media and quickly decomposes by oxidation. The results of this study should therefore be considered as the combined effect of the parent test item and all degradation products.

Reported statistics and error estimates:

Finney, D. J.: Probit analysis. Cambridge University Press, 3rd Edition, 1971

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a study according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), a 48 h EC50 = 1,1 mg/L for trimethylhydroquinone was determined.

Executive summary:

The acute toxicity of trimethylhydroquinone to Daphnia magna was investigated in a study according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under static conditions. The endpoint examined was immobilization. The 48 h EC50 was determined to be 1,1 mg/L based on nominal concentrations.

The testing/validity criteria laid down in the OECD guideline 202 are fulfilled. This study is regarded as reliable without restriction. Thus, a 48 h EC50 = 1,1 mg/L has to be taken into account for the assessment of trimethylhydroquinone.