2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

EC Number:

248-514-6

EC Name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Cas Number:

27538-10-9

Molecular formula:

C7H10O3

IUPAC Name:

2-ethyl-4-hydroxy-5-methylfuran-3(2H)-one

Specific details on test material used for the study:

Appearance: yellow liquid

Test animals / tissue source

Species:

cattle

Strain:

other: Bos primigenius Taurus (fresh bovine corneas)

Test system

Vehicle:

Hank's balanced salt solution

Controls:

yes, concurrent positive control

yes, concurrent negative control

Duration of treatment / exposure:

10 minutes

Duration of post- treatment incubation (in vitro):

2 hours

Results and discussion

In vitro

Results

Irritation parameter:

in vitro irritation score

Value:

154.24

Negative controls validity:

valid

Positive controls validity:

valid

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Conclusions:

This in vitro study was performed to assess corneal damage potential of Furanone Homo by quantitative measurements of changes in opacity and permeability in a bovine cornea. The test item Furanone Homo was brought onto the cornea of a bovine eye which previously had been incubated with cMEM without phenol red at 32 ± 1 °C for 1 hour and whose opacity had been determined. The test item was incubated on the cornea for 10 minutes at 32 ± 1 °C. After removal of the test item and 2 hours post-incubation, opacity and permeability values were measured. The test item was tested pure. Under the conditions of this test, the test item Furanone Homo induced serious eye damage on the cornea of the bovine eye. The calculated IVIS is 154.24. According to OECD Guideline no. 437 (Oct. 2017), a substance with an IVIS > 55 induces serious eye damage, that should be classified as UN GHS Category I.