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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
yes
Remarks:
This deviation has no impact on the validity of the study.
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Activated sludge was collected from the exit of the aeration tank, or near the exit from the tank, of a well-operated wastewater treatment plant receiving predominantly domestic sewage. On October 26th, 2017 activated sludge was sampled at Valence’s Waste treatment Plant. The sludge was settled and the liquid supernatant discarded. The concentrated sludge was then suspended in the mineral solution. These two steps were repeated twice.
Duration of test (contact time):
ca. 28 d
Initial conc.:
ca. 100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
The test item, at 100 mg/L, as the sole source of carbon and energy, was incubated in a buffer mineral salts medium which was inoculated with a mixed population of micro-organisms. The consumption of oxygen was determined from the change in volume or pressure (or a combination of the two) in the apparatus. Evolved carbon dioxide was absorbed in a solution of sodium hydroxide or another suitable absorbent. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of ThOD.
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 57.8
Sampling time:
28 d
Key result
Parameter:
BOD5
Value:
ca. 106.84 other: mg O2/L
Remarks on result:
other: a correction for the nitrification was performed.
Results with reference substance:
The BOD (Biological Oxygen Demand) of the reference substance was 178 mg O2/L.
The biodegradation of the reference substance was 92.9%.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
2-aminoethanol hydrobromide has a percentage of biodegradation of 57.8% at 28 days. It is therefore considered as not readily biodegradable.

Description of key information

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information