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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003-11-10 to 2004-02-26
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Details on sampling:
- Sampling method: At the beginning of the test (duplicate samples from each testmedium and from the control) and after 48 and 96 hours (duplicate samples from each test medium with surviving fish. All samples were taken from the approximate center of the aquaria without mixing of the test media.
- Sample storage conditions before analysis: stored in a deep-freezer (~-20°C) until analysis.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the 2 highest concentrations were prepared by dissolving the exact amount of T002251 into 5 L of test water using ultrasonic treatment for 15 minutes and intense stirring for 90 minutes at room t°. Adequate volume of these two test media were diluted with test water to prepare the lower concentrations.
- Controls: yes
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra fish
- Source: Zoohaus Schaub, CH-4410 Liestal, Switzerland)
- Length at study initiation (length definition, mean, range and SD): mean 4 cm, SD 0.2 cm
- Weight at study initiation (mean and range, SD): mean 0.58 g, SD 0.13 g
- Feeding during test: The fish were not fed during the test
- Food type: commercial dry fish food (Tetramin)
- Frequency: daily


ACCLIMATION
- Acclimation period: 1 week
- Acclimation conditions (same as test or not): same test water and temperature
- Type and amount of food: daily fed with commercial dry fish food
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no mortality

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
250 mg CaCO3/L
Test temperature:
22.0 - 23.0 °C
pH:
7.3 - 7.9
Dissolved oxygen:
7.4-9.1 mg /L
Salinity:
not applicable
Nominal and measured concentrations:
- Nominal concentrations: 1, 3.2, 10, 32, 100 mg/L.
- The analytically determined concentrations of the test item T002251 in the test medium were in the range from 87% to 101% of the nominal values. The results of the study can be based on the nominal concentrations.
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquaria
- Material, size, headspace, fill volume: capacity of 5 L
- Aeration: slightly aerated during the test period
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: less than 1.0 g/L

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Photoperiod: 16h light -to 8h dark photoperiod with a 30-minute transition period

TEST CONCENTRATIONS
- Spacing factor for test concentrations: x3.2
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: Yes, the choice of the enlarged spacing factor was done according to the results of the range-finding test
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 10-32
Details on results:
- Behavioural abnormalities:
* 10 mg/L: after 24h and during the entire test, fish were mainly at the bottom of the aquarium.
- Mortality of control: 0
- The 96-h LC100= 32 mg/L.
Reported statistics and error estimates:
Probit analysis or Moving average Interpolation could not be calculated due to the steep concentration-effect relationship.
LC50- values were determined as the geometric mean value of the 2 consecutive test concentrations with 0 and 100% mortality.
Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity of of T002251 was investigated in a 96-h static fish test folllwing the OECD guideline 203. The 96-h LC50 was determined to be 18 mg T002251/L. The results of the test can be considered reliable without restriction.

Description of key information

The study of Seyfried (2004), investigating the acute toxicity of T002251 in Danio rerio according to OECD guideline 203, was considered as the key study for endpoint coverage. A LC50 = 18 mg/L was obtained after an exposure of 96 hours in a static test in freshwater. Based on the results it can be considered that T002251 is harmful to fish.

Key value for chemical safety assessment

LC50 for freshwater fish:
18 mg/L

Additional information