1-chloro-4-nitrobenzene

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication/study report which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1986

Reference 2

Reference Type:

secondary source

Title:

1-CHLORO-4-NITROBENZENE CAS No: 100-00-5 SIDS Initial Assessment Report.

Author:

OECD

Year:

2002

Bibliographic source:

UNEP Publications

Materials and methods

Principles of method if other than guideline:

NEN 6502: Determination of chronic toxicity to Daphnia magna (1980)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-4-nitrobenzene

EC Number:

202-809-6

EC Name:

1-chloro-4-nitrobenzene

Cas Number:

100-00-5

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 4-chloronitrobenzene
- Analytical purity: > 98 %

Sampling and analysis

Analytical monitoring:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnids were fed with 3.0E+08 cells/L Chorella pyrenoidosa

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Hardness:

200 mg/L CaCO3

Test temperature:

20 °C

pH:

8.4

Details on test conditions:

- The population growth constant (Rm) of Daphnia magna was determined under semistatic conditions over a 21 days period, using 10 daphnids per concentration and one animal per jar containing 10 mL medium.
- The test was carried out in a room illuminated 12h/day.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

4.6 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality

Remarks on result:

other: 95% CL; 4.3-5.0

Effect concentrations 2

Duration:

21 d

Dose descriptor:

other: LRCT rm

Effect conc.:

1.8 mg/L

Nominal / measured:

nominal

Basis for effect:

other: population growth

Remarks on result:

other: LRCT = Lowest rejected concentration tested with effect (similar to LOEC); rm=intrinsic rate of natural increase

Effect concentrations 3

Duration:

21 d

Dose descriptor:

other: LRCT L

Effect conc.:

3.2 mg/L

Basis for effect:

growth

Remarks on result:

other: = Lowest rejected concentration tested with effect (similar to LOEC); L=mean length (L) of animals

Any other information on results incl. tables

Three chronic effects are presented.

1. Population growth:
LRCT (Rm)= Lowest rejected concentration tested which
significantly lowered the population growth constant (Rm)
after 21 days of exposure:
log LRCT(Rm) = 1.05 = 11 µmol/L.....LRCT (Rm)=1.8 mg/L 

2. Body length:

LRCT(L) = Lowest rejected concentration tested that significantly lowered the mean length (L) of animals after 21 days of exposure. log LRCT(L) = 1.31 = 20 µmol/L....LRCT(L)= 3.2 mg/L

3. Lethal concentration:

LC50 (21 days) = 4.6 (95 % confidence limits: 4.3 - 5.0) mg/L In another publication, Deneer et al. (Deneer, J.W. et al., QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum, Aquatic Toxicology 15, 83-98 (1989)), two of the authors presented the first two chronic effects but reported the third to be IC50 = 21 d immobilization concentration log IC50 = 1.46 = 28 µmol/L .......IC50 = 4.5 mg/L