2,2'-thiodiethanethiol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 Mar 1992 - 23 Dec 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-5 (Acute Dermal Irritation)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,3-bis((2-mercaptoethyl)thio)-1-propanethiol

EC Number:

411-290-7

EC Name:

2,3-bis((2-mercaptoethyl)thio)-1-propanethiol

Cas Number:

131538-00-6

Molecular formula:

C7 H16 S5

IUPAC Name:

2,3-bis((2-mercaptoethyl)thio)-1-propanethiol

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Thomae, Biberach, Germany
- Weight at study initiation: 2.19 ± 0.187 kg
- Housing: singly in wire mesh cages
- Diet (e.g. ad libitum): Altromin K, Type 2123, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Quarantine: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 2 °C
- Humidity (%): 45-65%
- Air changes (per hr): 12-15 /h
- Photoperiod (hrs dark / hrs light): 12 / 12

IN-LIFE DATES: From: 1992-07-28 To: 1992-08-18

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

0.5 mL

Duration of treatment / exposure:

4 h

Observation period:

14 days

Number of animals:

6 males

Details on study design:

TEST SITE
- Area of exposure: left or right of dorsal midline
- coverage: 2 x 3 cm gauze patch
- Type of wrap if used: fixomull

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 4 h

OBSERVATION TIME POINTS
0.5, 1, 6, 24, 48, 72 h; Days 4, 7, 10, and 14

SCORING SYSTEM:
- Method of calculation: Draize

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

1.72

Max. score:

4

Reversibility:

fully reversible within: Day 14

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0.22

Max. score:

4

Reversibility:

fully reversible within: Day 10

Irritant / corrosive response data:

see table below

Any other information on results incl. tables

Rabbit #	Time [h]	Erythema	Oedema
1	24	2	0
	48	2	0
	72	2	0
	average	2.0	0.0
2	24	1	0
	48	2	2
	72	1	2
	average	1.3	1.3
3	24	1	0
	48	3	0
	72	2	0
	average	2.0	0.0
4	24	1	0
	48	2	0
	72	1	0
	average	1.3	0.0
5	24	1	0
	48	1	0
	72	1	0
	average	1.0	0.0
6	24	2	0
	48	3	0
	72	3	0
	average	2.7	0.0
	Time [h]	Erythema	Oedema
average	24	1.33	0.00
score	48	2.17	0.33
	72	1.67	0.33
	24+48+72	1.72	0.22

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

DMPT is mildly irritating to skin, but does not meet the CLP criteria for classification as a skin irritant.