1,4-phenylene bis((4-hydroxyphenyl)methanone)

Endpoint summary

Description of key information

For 1,4-Bis (4 -hydroxy benzoyl) benzene short-term toxicity tests are available on green algae and aquatic invertebrates. Since no other toxicity studies are available for 1,4 -Bis(4 -hydroxy benzoyl) benzene, the results from the structural analogue

1,3-Bis(4 -hydroxy benzoyl) benzene are used instead (for details see Read-across justification as attached in section 13). For 1,4 -Bis(4 -hydroxy benzoyl) benzene a respiration test with activated sludge is available. In addition, a reproduction test with daphnia and an

early life stage toxicity test in fish test were conducted as part of a PMN in the US. Due to the low solubility of the test items in the test media, procedures were based on the test methods described in the OECD series on testing and assessment number 23 (2000).

Short-term toxicity data

- The acute toxicity of 1,4-Bis (4-hydroxy benzoyl) benzene to the freshwater flea Daphnia magna was investigated in a GLP compliant study according to OECD 202. No immobility was observed at any of the concentrations of 1,4-Bis (4-hydroxy benzoyl) benzene tested. The EC50 for immobilization (48h-EC50) for Daphnia magna was > 0.17 mg/L, measured concentration being considered to be the maximum soluble concentration of the test item in test medium at a loading rate of 100 mg/L. 

- A GLP compliant guideline study with 1,4-Bis (4-hydroxy benzoyl) benzene is available for the toxicity in algae (Raphidocelis subcapitata). Under the conditions of the study, no inhibition of growth rate or inhibition of yield was recorded at any of the concentrations of tested. The EC50 for both growth rate and yield inhibition (72h-ErC50and 72h-ErC50, respectively) was > 0.16, measured concentration being considered as the maximum soluble concentration of the test item in test medium at a loading rate of 100 mg/L.

- The effect of the structural analogue 1,3-Bis(4 -hydroxy benzoyl) benzene on the respiration of activated sewage sludge was assessed in a valid GLP study performed according to OECD TG 209. No toxicity was observed at a concentration of 1000 mg/L (NOEC). The EC50 was therefore >1000 mg/L.

Long-term toxicity data

-In the growth inhibition study with 1,4-Bis (4-hydroxy benzoyl) benzene on the algae species Raphidocelis subcapitata performed by Charles River (2018), no inhibition of growth rate or yield was recorded at any of the concentrations tested. The 72 -h-NOEC

for both growth rate and yield inhibition was ≥ 0.26 mg/L.

- For the structural analogue 1,3 -Bis(4 -hydroxy benzoyl) benzene, a GLP-compliant guideline study is available for the long-term toxicity in aquatic invertebrates (D. magna). Under the conditions of this study 1,3-Bis (4-hydroxy benzoyl) benzene did not affect reproduction, parental mortality and growth of Daphnia magna at the maximum solubility in test medium, i.e. at 0.16 mg/L, after 21 days of exposure (NOEC).

- A chronic fish study with the structural analogue 1,3 -Bis(4 -hydroxy benzoyl) benzene according to OECD 210 was performed with fathead minnow (Pimephales promelas) under flow-through conditions. Under the condition of the study

1,3-Bis (4-hydroxy benzoyl) benzene did not affect the hatching success, post-hatch survival or growth of the larvae at concentrations up to and including 0.10 mg/L (NOEC). Hence the LOEC was >0.10 mg/L.