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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-01-29 to 1990-02-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
EC Number:
228-800-7
EC Name:
2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
Cas Number:
6359-50-8
Molecular formula:
C22H25N2.Cl
IUPAC Name:
2-[2-(2,3-dihydro-2-methyl-1H-indol-1-yl)vinyl]-1,3,3-trimethyl-3H-indolium chloride
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
sampling at start of the test and at the time of 100% mortality or test end of respective test concentration

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test solutions were directly prepared

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- scientific name: Brachydanio rerio Hamilton-Buchanan
- Source: West Aquarium, Bad Lauterberg

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
21.0 - 22.3°C
pH:
7.3 - 8.0
Dissolved oxygen:
6.5 - 8.4 mg/L
Nominal and measured concentrations:
Nominal: 0 (control), 18.0, 32.4, 58.3, 105, 189 mg/L
Measured (geometric mean):
Details on test conditions:
Water quality criteria according to guideline
Light regime: 12/12 h
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
32.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
105 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 58.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other:
Remarks:
geometric mean LC0/LC100
Details on results:
- Behavioural abnormalities: none
- Other biological observations: dye accumulation on skin surface
- Mortality of control: 10%

Any other information on results incl. tables

Sublethal observations / clinical signs:

concentration
[mg/L]		 % mortality after
24h 48h 72h 96h
control 0 0 10 10
189.0 100  -  -  -
105.0 50 100  -  -
58.3 10 20 30 30
32.4 0 0 0 0
18.0 0 0 0 0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-h LC50 to Danio rerio was calculated to be 58.3mg test substance/L. The mortality at 58.3 mg/L is 30%, so the true LC50 is >= 58.3 mg/L. 58.3 mg/L is considered the worst case. The substance is acutely harmful to fish.