Registration Dossier
Registration Dossier
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EC number: 217-210-5 | CAS number: 1777-82-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981-01-19 to 1981-03-13
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Experimental study with details provided, under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not specified
- Principles of method if other than guideline:
- An ames test was conducted.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,4-dichlorobenzyl alcohol
- EC Number:
- 217-210-5
- EC Name:
- 2,4-dichlorobenzyl alcohol
- Cas Number:
- 1777-82-8
- Molecular formula:
- C7H6Cl2O
- IUPAC Name:
- (2,4-dichlorophenyl)methanol
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver microsomal metabolic activation system (S9)
- Test concentrations with justification for top dose:
- 62.5, 125, 250, 500, 1000 ug/plate
- Vehicle / solvent:
- - Vehicle: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- other: 2-aminofluorene, neutral red
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: semi-quantitative assay (plate)
- incubation time: 2 days
- Incubation temperature (°C): 37
- Counting: automatically, using Micro-Measurements E.T.A. colony counter
- Number of replicates: 3
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1535, TA1537, TA1538, TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Cytotoxicity was observed starting at 500 µg/plate without S9 mix and at 1000 µg/plate with S9 mix.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
