3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental rsults of read across chemicals

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below

Principles of method if other than guideline:

WoE report is prepared based on short term toxicity to aquatic invertebrates study.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
- Common name: Solvent Red 197
- Molecular formula:C23H19N5O
- Molecular weight: 381.437 g/mol
- Smiles : c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
- InChI : 1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
- Substance Type: Organic

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

1. The stock solution 100 g/L was prepared by dissolving clear yellow oily liquid in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.
2. The stock solution 100 g/l was prepared by dissolving white powder in acetone. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water.

Test organisms (species):

Daphnia magna

Details on test organisms:

In 1 and 2 nd study:
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 hr

Test temperature:

1. 20±1°C

pH:

1. Test: 8.2 (changed to 8.1 during test)
Control: 7.8 (no change during test)

2. Test: 7.9 (no change during test)
Control: 7.7 (Change to 7.8 during test)
Control + acetone: 7.9 changes to 7.8 during the test

Dissolved oxygen:

1. higher than 7.9 mg/L at the end of test
2. higher than 8.4 mg/L at the end of test

Nominal and measured concentrations:

1. 0, 0, 4, 10, 17, 30, 55 and 100 mg/l nominal concentrations
2. 0, 0, 0.5, 1, 2, 4, 8, 16 mg/l nominal concentrations

Details on test conditions:

1. TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

1. and 2. Potassium dichromate (K2Cr2O7)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

35.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: In 1 st study 95 % CI was 24.8-50 mg/l

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

10.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: For 2nd study 95 % CI was 9.9-11.9 mg/l

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Other details not known

Results with reference substance (positive control):

- Results with reference substance valid
1.EC50: 0.79 mg/L (24 hours)
2. - EC50: 0.88 mg/L (24 hours)

Reported statistics and error estimates:

EC50 was calculated using non linear regression by the software Prism 4.0

Validity criteria fulfilled:

yes

Conclusions:

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.
2.Based on the immobilisation of daphnia magna due to the exposure of chemical for 48 hrs, the median effective concentration EC50 was determine to be 10.9 mg/l.
3.After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l.

Executive summary:

Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/L was prepared by dissolving clear yellow oily liquid in DMSO. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted at various different nominal concentrations 0, 0, 4, 10, 17, 30, 55 and 100 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

In the second study for another read across chemical toxicity was measured. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving white powder in acetone. Test solutions of required concentrationas were prepared by mixing the stock solution of the test sample with reconstituted test water. Acetone used as a solvent because of limited solubility of tested sample in water. 0, 0, 0.5, 1, 2, 4, 8, 16 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be10.9 mg/L with the confidence interval of 9.9 - 11.9 mg/l on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Study was conducted to determine the nature and effects of test chemical on the growth of aquatic invertebrate. Study was conducted according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under the static system by providing the exposure period of 48 hrs. After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l. based on the EC50, chemical was consider as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Description of key information

1. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobiity inhibition effects in a 48 hour study.

2.Based on the immobilisation of daphnia magna due to the exposure of chemical for 48 hrs, the median effective concentration EC50 was determine to be 10.9 mg/l.

3.After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l.

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic as the value ranges from 10.9 -35.2 mg/l and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

35.2 mg/L

Additional information

Short term toxicity of 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/L was prepared by dissolving clear yellow oily liquid in DMSO. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Test conducted at various different nominal concentrations 0, 0, 4, 10, 17, 30, 55 and 100 mg/l. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be 35.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

In the second study for another read across chemical toxicity was measured. Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs. The stock solution 100 g/l was prepared by dissolving white powder in acetone. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. Acetone used as a solvent because of limited solubility of tested sample in water. 0, 0, 0.5, 1, 2, 4, 8, 16 mg/l nominal concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance in Daphnia magna was determined to be10.9 mg/L with the confidence interval of 9.9 - 11.9 mg/l on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, it can be concluded that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Similarly short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals. Study was conducted to determine the nature and effects of test chemical on the growth of aquatic invertebrate. Study was conducted according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) under the static system by providing the exposure period of 48 hrs. After the incubation period of 48 hrs of test organism with the test chemical, effect were observed at 11 mg/l. based on the EC50, chemical was consider as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (52372 -39 -1), it can be concluded that the test chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.