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Description of key information

In Acute oral toxicity,LD50 value for target substance 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) was considered to be 4100 mg/kg bw in rats and 6200 mg/kg bw in mouse,and for different studies available on functionally similar read across substance was considered to be 6000 mg/kg bw in rats and 4360 mg/kg bw in mouse. All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7)cannot be classified for acute oral toxicity.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Justification for type of information:
Data is from Danish QSAR
Qualifier:
equivalent or similar to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Prediction is done by using Danish QSAR database
GLP compliance:
not specified
Test type:
other: Not specified
Limit test:
no
Specific details on test material used for the study:
- Name of test material: 2,3-bis[(4-methylbenzoyl)oxy]succinic acid
- IUPAC name: (+)-Di-p-toluoyl-D-tartaric Acid
- Molecular formula: C20H18O8
- Molecular weight: 386.3542 g/mole
- Smiles : Cc1ccc(cc1)C(=O)O[C@@H]([C@H](OC(=O)c2ccc(C)cc2)C(=O)O)C(=O)O
- Inchl: 1S/C20H18O8/c1-11-3-7-13(8-4-11)19(25)27-15(17(21)22)16(18(23)24)28-20(26)14-9-5-12(2)6-10-14/h3-10,15-16H,1-2H3,(H,21,22) (H,23,24)/t15-,16-/m0/s1
- Substance type: Organic
- Physical state: Solid powder (white)
Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
Not specified
Route of administration:
oral: unspecified
Vehicle:
not specified
Details on oral exposure:
Not specified
Doses:
4100 mg/kg bw
No. of animals per sex per dose:
Not specified
Control animals:
not specified
Details on study design:
Not specified
Statistics:
Not specified
Preliminary study:
Not specified
Sex:
not specified
Dose descriptor:
LD50
Effect level:
4 100 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50% mortality was observed
Mortality:
Not specified
Clinical signs:
Not specified
Body weight:
Not specified
Gross pathology:
Not specified
Other findings:
Not specified
Interpretation of results:
other: not classified
Conclusions:
Based on the QSAR prediction done using the Danish (Q)SAR Database, the LD50 was estimated to be 4100 mg/kg bw on rat for test substance 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) orally.
Executive summary:

Based on the QSAR prediction done using the Danish (Q)SAR Database, the LD50 was estimated to be 4100 mg/kg bw on rat for 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) having Reliability Index: 0.56 (moderate prediction quality).

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
4 100 mg/kg bw
Quality of whole database:
Data is Klimisch 2 and from Danish (Q)SAR Database

Acute toxicity: via inhalation route

Endpoint conclusion
Endpoint conclusion:
no study available

Acute toxicity: via dermal route

Endpoint conclusion
Endpoint conclusion:
no study available

Additional information

Acute Oral Toxicity: 

In different studies, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experimental data in rodents, i.e. most commonly in rats for 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) along with the study available on the functionally similar read across substance. The studies are summarized as below –

The study mentioned in Danish (Q)SAR Database (2018) for the target chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) was for acute oral toxicity. Based on the QSAR prediction done using the Danish (Q)SAR Database, the LD50 was estimated to be 4100 mg/kg bw on rat for 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) having Reliability Index: 0.56 (moderate prediction quality).

The another study mentioned in Danish (Q)SAR Database (2018) for the target chemical 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) was for acute oral toxicity. Based on the QSAR prediction done using the Danish (Q)SAR Database, the LD50 was estimated to be 6200 mg/kg bw on mouse for 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) having Reliability Index: 0.7 ( moderate prediction quality).

Both these studies are again supported with the functionally similar read across substance. Acute oral toxicity study was performed in rats using test chemical.50% mortality was observed at dose 6000 mg/kg bw. Hence,LD50 value was considered to be 6000 mg/kg bw,when rats were treated with test chemical orally.

The above studies were further supported with the another functionally similar read across substance. Acute oral toxicity study was performed in mouse using test chemical.50% mortality was observed at dose 4360 mg/kg bw. Hence,LD50 value was considered to be 4360 mg/kg bw,when mouse were treated with test chemical orally.

Thus, based on the above summarised studies on 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) and it’s functionally similar read across substance, it can be concluded that LD50 value is >2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) cannot be classified for acute oral toxicity.

Justification for classification or non-classification

Based on the above experimental studies on 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) and it’s functionally similar read across substances, it can be concluded that LD50 value is >2000 mg/kg bw for acute oral toxicity.Thus, comparing this value with the criteria of CLP regulation, 2,3-bis[(4-methylbenzoyl)oxy]succinic acid (32634-68-7) cannot be classified for acute oral toxicity.