1-Propanaminium, 3,3',3''-[phosphinylidynetris(oxy)]tris[N-(3-aminopropyl)-2-hydroxy-N,N-dimethyl-, N,N',N''-tri-C6-18 acyl derivs. trichlorides

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

96 hours

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Performed to guidelines, but no supporting analysis
The substance is supplied in water and is considered stable,

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Specific details on test material used for the study:

Tested as 7% actives product in water for supply

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Water

Test organisms (species):

other: turbot

Details on test organisms:

Juvenile fish used

Test type:

static

Water media type:

saltwater

Total exposure duration:

96 h

Test temperature:

14 C

pH:

7.8 - 8.0

Dissolved oxygen:

> 83%

Salinity:

Natural sea water

Nominal and measured concentrations:

The dose level was set at the concentration known to be toxic to other organisms under animal welfare rules.
Maximum concentration 4 mg/l as 7% actives (0.28 mg/l actives)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

7% actives

Basis for effect:

mortality (fish)

Details on results:

No mortality seen at 4 mg/l of the 7% solution (ie 0.28 mg/l)
The dose level was set at the concentration known to be toxic to other organisms under animal welfare rules.

Validity criteria fulfilled:

yes

Conclusions:

The results confirm that fish are less sensitive to the substance than other organisms tested.

Description of key information

A study performed on turbot confirmed that fish are less sensitive than other organisms. The dose level was set at the concentration known to be toxic to other organisms under animal welfare rules.

The read-across to the betaine is conisdered justified as the phosphate ester is considered likely to metabolise to form products that include phosphated betaines.

Evidence shows that fish are more tolerant to the class of substance than algae and further animal testing is not justifed

Acute toxicity is predicted to be in the range of 1 - 10 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2 mg/L

Additional information

The headline result is from a weight of evidence study on zebra fish