Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 03 June 2016 to 11 November 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report Date:
2016

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
2008
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
Version / remarks:
1995
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 830.7550 (Partition Coefficient, n-octanol / H2O, Shake Flask Method)
Version / remarks:
1996
Deviations:
no
GLP compliance:
yes
Type of method:
flask method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Appearance: white solid
- Storage conditions: in refrigerator (2-8°C) protected from light

Study design

Analytical method:
other: ultra-performance liquid chromatography

Results and discussion

Partition coefficient
Key result
Type:
log Pow
Partition coefficient:
0.25
Temp.:
20 °C
pH:
>= 3.6 - <= 3.7
Details on results:
- The analysed concentration of the stock solution was 1.03 g/L; nominal quantity in each test sample was 2.10 mg. Recoveries: 100% to 02%.
- No test item was detected in the pretreated samples from the blank mixture with the exception of first analysis of n-octanol phase pretreated sample from the blank mixture. It was considered that it has no significant effect on the results of the test samples since in the duplicate injection no test item was found.
- The log Pow values were within the criterion range of ± 0.3 log units (see table below)

Any other information on results incl. tables

Table: Shake-flask results

  

Ratio
n-octanol : water

[v:v]

Analysed

concentration [g/L]

Pow

log Pow

pH

n-octanol

water

individual

mean

 

 

 

 

 

 

 

1:1

0.227

0.124

1.83

1.83

0.26

3.6

 

0.223

0.122

1.83

 

0.26

3.6

 

 

 

 

 

 

 

1:2

0.254

0.134

1.90

1.92

0.28

3.6

 

0.256

0.132

1.94

 

0.29

3.6

 

 

 

 

 

 

 

2:1

0.200

0.128

1.57

1.62

0.20

3.6

 

0.199

0.119

1.67

 

0.22

3.7

 

 

 

 

 

 

 

Mean

1.8

 

0.25

 

Standard deviation

0.14

 

 

 

 

 

Applicant's summary and conclusion

Conclusions:
The log Pow at 20°C was determined to be 0.25.
Executive summary:

The partition coefficient of the substance was determined using the shake-flask method in a GLP study according to EC A.8, OECD 107 and OPPTS 830.7550. Quantitative analysis was performed by UPLC. Recoveries were in the range of 100 -102%. The pH of the aqueous phases was 3.6 -3.7. The log Pow at 20°C was determined to be 0.25.