Reaction product of the esterification of diglycerol with decanoic acid (Old name: 1,2,3-Propanetriol, homopolymer, decanoate)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008.05.20-2008.05.22

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Remarks:

The chemical analysis was carried out as accredited analysis but not in compliance with the GLP principles.

Specific details on test material used for the study:

Batch no. 512051
Manufacturing data: December 2005
Origin: Dr. Straetmans Chemische Produkte GmbH, Germany
Form: Almost colourless viscous liquid

Details on test solutions:

The test product is not soluble but dispersible in water.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus
The strain has been collected in Langedam, Birkerød, Denmark and cultured at DHI since 1979. The culture is fed with Pseudokirchneriella subcapitata three times a day.

Test type:

semi-static

Water media type:

other: ISO-medium

Total exposure duration:

48 h

Hardness:

252 mg/L expressed as CaCO3

Test temperature:

20.1 ± 0.1°C

pH:

pH 7.7

Nominal and measured concentrations:

Nominal concentrations: 0 (control); 5.0; 10; 20; 50; and 200 mg/L

Details on test conditions:

All test solutions were renewed after 24 hours of exposure.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate, analytical grade (Merck 4864)

Key result

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

6.01 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

15.1 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Results with reference substance (positive control):

A 24-h acute toxicity test on the reference substance was performed at the following concentrations: 0.20; 0.40; 0.70; 1.0; 1.4 and 2.0 mg/L.
The EC50 value for potassium dichromate was 0.99 mg/L based on nominal concentrations.

Reported statistics and error estimates:

The EC10 and EC50 values for the acute toxicity of the test product and reference substance were calculated by use of a standard procedure probit analysis.

Validity criteria fulfilled:

yes

Conclusions:

The results from the 48-hour toxicity test showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.

Executive summary:

The toxicity of Dermosoft DGMC to aquatic invertebrates was studied in a test performed according to the OECD guideline no. 202 and following GLP. The test was carried out as a 48-hour semi-static test, i.e. all test solutions were renewed after 24 hours of exposure.

Dermosoft DGMC was tested in the nominal concentrations: 0 (control); 5.0; 10; 20; 50; 100 and 200 mg/L.

The chemical analyses of NVOC showed that the average recovery for the total exposure period was very high in all subsamples collected. The mean recovery was ≥80% in all test solutions from 5 mg/mL to 200 mg/L during the test. The statistical calculations were thus made on basis of the nominal concentrations.

The data analysis showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.

Description of key information

The results from the 48-hour toxicity test showed that the EC10 (48h) was 6.01 mg/L (3.55 -8.19) and EC50 (48h) was 15.1 mg/L (11.7 -19.5) based on nominal concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

15.1 mg/L