Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From January 08, 2018 to March 21, 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test material information:
Composition 1
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
80-110 mg/L as CaCO3
Test temperature:
19.1-19.7°C
pH:
7.2-8.2
Dissolved oxygen:
8.9-9.2 mg/L
Nominal and measured concentrations:
Nominal concentration: 100 mg/L
Details on test conditions:
- Test vessel: ~250 mL glass beakers
- Test volume: 200 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark with a 30 minute transition period
- Light intensity: 528-566 lux
Reference substance (positive control):
yes
Remarks:
Zinc sulphate heptahydrate (CAS No.: 7446-20-0)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
48-hr LC50 of zinc sulphate heptahydrate was 0.59 mg Zn2+/L

The 0% of control organisms were immobilised and the dissolved oxygen concentration at the end of the test was 8.9-9.0 mg O2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test. At the end of the test, all test solutions were clear and colorless.

 

Table 1. Number and Percentage of Daphnia magna Immobilised after 24-hr Exposure

Nominal Concentration of Test Item

(mg/L)

Mean ± SD

(%)

Replicate A

(# / %)

Replicate B

(# / %)

Replicate C

(# / %)

Replicate D

(# / %)

Negative Control

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

100

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

Table 2. Number and Percentage of Daphnia magna Immobilised after 48-hr Exposure

Nominal Concentration of Test Item

(mg/L)

Mean ± SD

(%)

Replicate A

(# / %)

Replicate B

(# / %)

Replicate C

(# / %)

Replicate D

(# / %)

Negative Control

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

100

0 ± 0

0 / 0

0 / 0

0 / 0

0 / 0

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of I850 was >100 mg/L.
Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1738 which is based on the SOP for the OECD 202 (BBY2SOP-00017) and OECD 202 (OECD, 2004).The results of this OECD 202 test for I850 show that all validity criteria were met. Based on the nominal concentrations of I850, the 48-hr EC50 was >100 mg/L.

Description of key information

The 48-hr EC50 of I850 was >100 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

The test was considered valid as 0% of control organisms were immobilised and the minimum dissolved oxygen concentration at the end of the test was 8.8-9.0 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. The measured concentrations of I850 were within 80-100% of their nominal concentrations. Based on the nominal concentrations of I850, the 48-hr EC50 was >100 mg/L.