Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental result of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mention below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical:
1 and 2

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes
- Molecular formula : C18H11NO8S2.Al
- Molecular weight : 460.39 g/mol
- Smiles notation : O=C1c2ccccc2C(=O)C1=C1C=Cc2cc3c4cc2N{-}1.[Al]{3+}(.O{-}S3(=O)=O).O{-}S4(=O)=O
- Substance type : Organic
- Physical state : Solid

Analytical monitoring:

yes

Details on sampling:

Concentrations: 100, 80, 60, 40, 20, and 10 mg/L
- Sample storage conditions before analysis: effluent samples were stored in amber glass bottles (2.0 L), transported to the laboratory well before 48-h in a lightproof ice chest, stored under refrigeration
(at 4 °C) in darkness until analysis

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

1. TEST ORGANISM
- Common name: Daphnia magna
- Source: laboratory stock culture
- Age at study initiation: 24 hr old neonatal
- Feeding during test : 24-h before experiment
- Food type: Selenastrum sp. and yeast powder

2. - Common name:
- Strain: Daphnia magna
- Source: Daphtoxkit F™ magna, a commercial kit that provides daphnids in the form of dormant eggs (ephippia)
- Age at study initiation : 24 h old neonates
- Feeding during test: prior 2 days of test
- Food type: lyophilized spirulina

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

1. 180.0 mg/l

Test temperature:

1. 25 ± 2.0 °C
2. 20±1 °C

pH:

1. 7.8

Nominal and measured concentrations:

2. 100 mg/L

Details on test conditions:

1. Test vessel: 15 mL glass test tube

Reference substance (positive control):

yes

Remarks:

The experiment was validated with positive control test using reference substance potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: For 1 study

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4.271 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: For 2nd study: confidence interval (C.I.)-3.680-4.957

Results with reference substance (positive control):

2 .potassium dichromate : 0.6-2.1 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

1.Test material when subjected to D. magna for 48 hrs the LC50 value was observed to be in the range 3.6-6.0 mg/l by chemical exposure.
2. The effect concentration of test material against Daphnia magna after 48 hrs was observed to be 4.271 mg/l

Executive summary:

Short term toxicity of Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes (94891 -32 -4) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Daphnia sp. Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment. Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L. After the exposure of test chemical for 24 hrs, the LC50 value was determine to be 6 mg/l and the 48hrs LC50 was determine in the range of 3.6 -6 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

Similar study was performed for another structurally and functionally similar read across chemicals. The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test. Test conducted under the static system for 48 hrs. The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957 mg/l. Based on the LC50, chemical was consider as toxic and can be consider to ba classified as aquatic chronic 2 as per the CLP classification criteria.

Thus based on the overall studies for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.

Description of key information

1.Test material when subjected to D. magna for 48 hrs the LC50 value was observed to be in the range 3.6-6.0 mg/l by chemical exposure.

2. The effect concentration of test material against Daphnia magna after 48 hrs was observed to be 4.271 mg/l

Thus based on the overall studies for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6 mg/L

Additional information

Short term toxicity of Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)-1,2-dihydro-6,7-quinolinedisulfonate complexes (94891 -32 -4) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates from journal. Daphnia sp. Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment. Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L. After the exposure of test chemical for 24 hrs, the LC50 value was determine to be 6 mg/l and the 48hrs LC50 was determine in the range of 3.6 -6 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 2 as per the CLP classification criteria.

 

Similar study was performed for another structurally and functionally similar read across chemicals. The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test. Test conducted under the static system for 48 hrs. The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957 mg/l. Based on the LC50, chemical was consider as toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

 

Thus based on the overall studies from peer reviewed journal for the structurally and functionally similar read across chemical for Aluminum, 2-(1,3-dihydro-1,3-dioxo-2H-inden-2-ylidene)- 1,2-dihydro-6,7- quinolinedisulfonate complexes (94891-32-4), it can be concluded that the test chemical was toxic and classifies as aquatic chronic 2 as per the CLP classification criteria.