Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Documented parameters are in accordance with method.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 3.7-Dimethyl-6-octen-1-al
(Citronellal)
Purity: no data
Water solubility: insoluble

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Municipal activated sludge from laboratory wastewater treatment plant fed with municipal sewage and synthetic wastewater.
- final dry solids concentration: 5 g/l

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: 100 and 1000 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorphenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Any other information on results incl. tables

respiration rate after 30 min of incubation

nominal test / respiration / respiration / respiration /
concentration / rate / inhibition / promotion /
(mg/l) / (mg/l*h) / (%) / (%) /
100 / 35 / 0 / 20 /
1000 / 17 / 43 / 0 /

Applicant's summary and conclusion