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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990
Reference Type:
other: recalculation
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German standard DIN 38412, Part 9
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Citronellal
EC Number:
203-376-6
EC Name:
Citronellal
Cas Number:
106-23-0
Molecular formula:
C10H18O
IUPAC Name:
3,7-dimethyloct-6-enal
Details on test material:
- Name of test material (as cited in study report): 3.7-Dimethyl-6-octen-1-al
(Citronellal)
Purity: no data

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20 °C
Nominal and measured concentrations:
Nominal test concentrations: 0 (control), 0.78, 1.58, 3.125, 6.25, 12.5, 25, 50, 100 mg/l
Details on test conditions:
TEST SYSTEM
- Fill volume: 100 ml


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guidelines.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
13.33 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
4.52 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
6.74 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
1.26 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
Neither growth promotion, induced by the test substance, nor auto fluorescence were observed during the test.
EC90(72h) = 20 mg/l

Any other information on results incl. tables

During the 72 h exposure to citronellal, fluorescence values were as  follows (average of 4 replicates)*:

Nominal test / fluorometer values after x hours /
concentration
(mg/l) / 0 / 24 / 48 / 72 /
Control / 34 / 103 / 402 / 1176 /
Solvent / 37 / 119 / 463 / 1241 /
0.78 / 36 / 105 / 437 / 1185 /
1.56 / 36 / 104 / 383 / 1151 /
3.125 / 36 / 97 / 336 / 997 /
6.25 / 36 / 79 / 245 / 720 /
12.5 / 35 / 56 / 125 / 236 /
25.0 / 37 / 30 / 36 / 48 /
50.0 / 35 / 21 / 16 / 8 /
100.0 / 30 / 6 / 1 / 1 /

*10000 cells/ml were introduced at the test start.


ECx values (in mg/l) after x hours of exposure

/ 24 h / 48 h / 72 h /

EC20 / 3.44 / - / 3.18 /

EC50 / 11.6 / - / 7.54 /

EC90 / 80.4 / - / 19.9 /

No chemical dose verification was performed. All values refer to nominal test concentrations of Citronellal.

Applicant's summary and conclusion