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Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was performed according to German national standards DIN.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German standard DIN 38 412, part L15.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Citronellal
EC Number:
203-376-6
EC Name:
Citronellal
Cas Number:
106-23-0
Molecular formula:
C10H18O
IUPAC Name:
3,7-dimethyloct-6-enal
Details on test material:
- Name of test material (as cited in study report): 3.7-Dimethyl-6-octen-1-al
(Citronellal)
- Analytical purity: ca. 98 %
Water solubility: insoluble (according to the sponsor)
- Lot/batch No.: B 1160

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed in to the aquaria. A control (test water without test substance) was tested in parallel.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 6.7 cm (5.7 - 7.2 cm)
- Weight at study initiation (mean and range, SD): 2.9 g (1.5 - 4.4 g)
Housing: oil-free aerated and charcoal filtered tap water, flow-through system Water temperature: 20-21 °C Duration of housing: ca. 1 month. Mortality during last 2 weeks of housing: ca. 1.2 %. Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride ,withdrawal of food 1 day before and during exposure
Corpulence factor of the batch: 1.0


ACCLIMATION
- Acclimation period:3 days
- Type and amount of food: growing feed (ad libitum);
- Health during acclimation (any mortality observed):no mortality observed

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/l
Test temperature:
21 °C
pH:
7.4 - 7.7
Dissolved oxygen:
7.2 - 8.5 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
10.0, 21.5, 46.4, 100.0 and control
Details on test conditions:
TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Type (delete if not applicable): open / closed
- Fill volume: 10 l
- Aeration: slight, continuous aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 2.9 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/l CaCl2 * 2 H2O 123.3 mg/l MgSO4 + 7 H2O 63.0 mg/l NaHCO3 5.5 mg/l KCl
- Ratio Na/K ions: 10 : 1
- Acid capacity: 0.8 mmol/l:
- Ca/mg ratio: 4 : 1

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours (day - night regime)
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
ca. 22 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
46.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No undissolved test substance was visible in the aquaria.
Results with reference substance (positive control):
The LC50 (48 h) of the positive control chloroacetamide was about 32 mg/l, which corresponds to the normal sensitivity of the fish.
Reported statistics and error estimates:
Probit analysis

Any other information on results incl. tables

Sublethal observations / clinical signs:
During the 96 h exposure to citronellal, mortalities were as follows:

Nominal test / / Cumulative mortality (%) after /
concentration / 1 h / 4 h / 24 h / 48 h / 72 h / 96 h /
(mg/l) / / / / / / /
Control / 0 / 0 / 0 / 0 / 0 / 0 /
10.0 / 0 / 0 / 0 / 0 / 0 / 0 /
21.5 / 0 / 10 / 40 / 40 / 40 / 40 /
46.4 / 0 / 100 / 100 / 100 / 100 / 100 /
100.0 / 0 / 100 / 100 / 100 / 100 / 100 /


Maximum test concentration causing no mortality: 10.0 mg/l.
Minimum test concentration causing 100% mortality: 46.4 mg/l.

The 96-hour EC50 calculated by probit analysis was about 21.5 mg/l.

Symptoms of intoxication:

Nominal test Symptoms of intoxication after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96 /
(mg/l)
Control - / - / - / - / - / - /
10.0 - / - / - / - / - / - /
21.5 - / N / A / A / - / - /
46.4 T / + / + / + / + / + /
100.0 NT/ + / + / + / + / + /

Definitions of Symptoms:
-: No symptoms
A: Apathy
N: Narcotic-like state
T: Tumbling
+: All fish dead

All values refer to nominal concentrations of Citronellal.
No analytical dose-verification of the test item was carried out.

Applicant's summary and conclusion