Registration Dossier

Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13 - 21 February 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Version / remarks:
1995
Deviations:
yes
Remarks:
see principles of method if other than guideline
Qualifier:
according to
Guideline:
EU Method A.6 (Water Solubility)
Version / remarks:
Regulation (EC) No 440/2008
Deviations:
yes
Remarks:
see principles of method if other than guideline
Principles of method if other than guideline:
The temperature in the equilibration phase ranged from 20.0 to 20.6 °C, with a mean of 20.3 °C and was thus out of the range of 20 ± 0.5 °C. This should have had no influence on the study, as the deviation was minor and the overall mean was in the range given by the guideline.
GLP compliance:
yes (incl. certificate)
Type of method:
flask method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
impurity
Test material form:
solid: crystalline
Remarks:
crystalline powder
Details on test material:
Batch No: 11679700
Storage: at room temperature, protected from light

Results and discussion

Water solubility
Key result
Water solubility:
3.51 g/L
Conc. based on:
test mat. (dissolved fraction)
Temp.:
20 °C
pH:
3.1
Details on results:
Preliminary test
The preliminary test indicated a water solubility of 1 to 10 g/L.

Main test
The results are given in the table in the next section. The water solubility was determined as the mean of the replicates analysed with HPLC.
No significant variation of the concentrations and pH values was observed in the three respective test vessels after one, two and three days of incubation at 30 °C (followed by 24 h equilibration at ca. 20 °C).
The test is satisfactory, if the concentrations of the three vessels do not differ by more than 15%.

Any other information on results incl. tables

HPLC determinations:

 Test vessel  pH  Analysed concentration A (g/L)   Analysed concentration B (g/L)   Analysed concentration C (g/L)  Mean (g/L)
 No. 1  3.1  3.60  3.57  3.60  3.59
 No. 2  3.0  3.44  3.42  3.44  3.43
 No. 3  3.1  3.47  3.55  3.50  3.50
         Overall Mean  3.51

The concentrations of the three vessels differed by 4% (validity criterion given by OECD is met).

Applicant's summary and conclusion

Conclusions:
The water solubility of Adenosine-5’-monophosphate (AMP) (CAS no. 61-19-8) was determined according to a modified TG OECD 105 design.
The water solubility of the test item at 20°C was 3.51 g/L. The pH of the aqueous samples was 3.1.
Executive summary:

The water solubility of Adenosine-5’-monophosphate (AMP, CAS no. 61-19-8) was determined according to TG OECD 105. Several test vessels containing ultra-pure water mixed with test item in great excess (initial amount of test item about 50 g per liter water), were incubated and stirred for different periods of time at about 30 °C (saturation phase), and then transferred to ca. 20 °C for 24 h (equilibration phase). Undissolved test item was subsequently separated by centrifugation. The concentration of test item in the clear aqueous phase was determined with HPLC.

The measured concentrations were as follows:

3.59 g/L after one day at 30 °C, followed by 24 h at 20 °C, pH 3.1

3.43 g/L after two days at 30 °C, followed by 24 h at 20 °C, pH 3.0

3.50 g/L after three days at 30 °C, followed by 24 h at 20 °C, pH 3.1.

No significant variation of the concentrations and pH values was observed in the three respective test vessels after one, two and three days of incubation at 30 °C (followed by 24 h equilibration at ca. 20 °C).

The water solubility of Adenosine-5’-monophosphate (AMP), calculated as the mean of all three test vessels, is 3.51 g/L at a pH of 3.1 and ca. 20 °C. The validity criterion was fulfilled.