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EC number: 200-500-0 | CAS number: 61-19-8
Water solubility
Administrative data
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 - 21 February 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Version / remarks:
- 1995
- Deviations:
- yes
- Remarks:
- see principles of method if other than guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Version / remarks:
- Regulation (EC) No 440/2008
- Deviations:
- yes
- Remarks:
- see principles of method if other than guideline
- Principles of method if other than guideline:
- The temperature in the equilibration phase ranged from 20.0 to 20.6 °C, with a mean of 20.3 °C and was thus out of the range of 20 ± 0.5 °C. This should have had no influence on the study, as the deviation was minor and the overall mean was in the range given by the guideline.
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
Test material
- Reference substance name:
- Adenosine phosphate
- EC Number:
- 200-500-0
- EC Name:
- Adenosine phosphate
- Cas Number:
- 61-19-8
- Molecular formula:
- C10H14N5O7P
- IUPAC Name:
- adenosine phosphate
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- Oxidane
- Test material form:
- solid: crystalline
- Remarks:
- crystalline powder
- Details on test material:
- Batch No: 11679700
Storage: at room temperature, protected from light
Constituent 1
impurity 1
Results and discussion
Water solubility
- Key result
- Water solubility:
- 3.51 g/L
- Conc. based on:
- test mat. (dissolved fraction)
- Temp.:
- 20 °C
- pH:
- 3.1
- Details on results:
- Preliminary test
The preliminary test indicated a water solubility of 1 to 10 g/L.
Main test
The results are given in the table in the next section. The water solubility was determined as the mean of the replicates analysed with HPLC.
No significant variation of the concentrations and pH values was observed in the three respective test vessels after one, two and three days of incubation at 30 °C (followed by 24 h equilibration at ca. 20 °C).
The test is satisfactory, if the concentrations of the three vessels do not differ by more than 15%.
Any other information on results incl. tables
HPLC determinations:
| Test vessel | pH | Analysed concentration A (g/L) | Analysed concentration B (g/L) | Analysed concentration C (g/L) | Mean (g/L) |
| No. 1 | 3.1 | 3.60 | 3.57 | 3.60 | 3.59 |
| No. 2 | 3.0 | 3.44 | 3.42 | 3.44 | 3.43 |
| No. 3 | 3.1 | 3.47 | 3.55 | 3.50 | 3.50 |
| Overall Mean | 3.51 |
The concentrations of the three vessels differed by 4% (validity criterion given by OECD is met).
Applicant's summary and conclusion
- Conclusions:
- The water solubility of Adenosine-5’-monophosphate (AMP) (CAS no. 61-19-8) was determined according to a modified TG OECD 105 design.
The water solubility of the test item at 20°C was 3.51 g/L. The pH of the aqueous samples was 3.1. - Executive summary:
The water solubility of Adenosine-5’-monophosphate (AMP, CAS no. 61-19-8) was determined according to TG OECD 105. Several test vessels containing ultra-pure water mixed with test item in great excess (initial amount of test item about 50 g per liter water), were incubated and stirred for different periods of time at about 30 °C (saturation phase), and then transferred to ca. 20 °C for 24 h (equilibration phase). Undissolved test item was subsequently separated by centrifugation. The concentration of test item in the clear aqueous phase was determined with HPLC.
The measured concentrations were as follows:
3.59 g/L after one day at 30 °C, followed by 24 h at 20 °C, pH 3.1
3.43 g/L after two days at 30 °C, followed by 24 h at 20 °C, pH 3.0
3.50 g/L after three days at 30 °C, followed by 24 h at 20 °C, pH 3.1.
No significant variation of the concentrations and pH values was observed in the three respective test vessels after one, two and three days of incubation at 30 °C (followed by 24 h equilibration at ca. 20 °C).
The water solubility of Adenosine-5’-monophosphate (AMP), calculated as the mean of all three test vessels, is 3.51 g/L at a pH of 3.1 and ca. 20 °C. The validity criterion was fulfilled.
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