Barium phosphinate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from authoritative database.

Data source

Reference

Reference Type:

other: Authoritative database

Title:

Ocular irritation study of test substance

Author:

U. S. National Library of Medicine

Year:

2018

Bibliographic source:

HSDB (Hazardous Substances Data Bank); US national Library of Medicine reviewed by SRC, 2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 870.2400 (Acute Eye Irritation)

Principles of method if other than guideline:

The potential of test chemical to induce eye irritation was assessed in 9 rabbits according to methods similar to OPPTS 870.2400 and OECD 405 guidelines.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium phosphinate

EC Number:

231-669-9

EC Name:

Sodium phosphinate

Cas Number:

7681-53-0

Molecular formula:

H3O2P.Na

IUPAC Name:

Sodium hypophosphite

Test material form:

solid

Details on test material:

- Name of test material: sodium phosphinate
- IUPAC name: Sodium hypophosphite
- Molecular formula: NaO2P
- Molecular weight: 87.9778 g/mole
- Smiles notation: [Na+].[O-]P=O
- InChl: 1S/Na.H3O2P/c;1-3-2/h;3H2,(H,1,2)/q+1;/p-1
- Substance type: Inorganic
- Physical state: Solid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

not specified

Test system

Vehicle:

not specified

Controls:

other: The other untreated eye served as control.

Amount / concentration applied:

100 mg

Duration of treatment / exposure:

72 hours

Observation period (in vivo):

24, 48 and 72 hours after the administration and then daily until reversibility of the ocular reactions.

Number of animals or in vitro replicates:

3 (Male/female)

Details on study design:

TEST SITE
- Area of exposure: one eye
REMOVAL OF TEST SUBSTANCE
- Washing (if done): For 6 rabbits, the eyes were not rinsed after administration of the test item while a washing procedure was applied for 3 other animals.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

chemosis score

Remarks:

For rinsed eye

Basis:

mean

Time point:

72 h

Score:

0.33

Reversibility:

not specified

Remarks on result:

no indication of irritation

Results 2

Irritation parameter:

conjunctivae score

Remarks:

For rinsed eye

Basis:

mean

Time point:

72 h

Score:

0.89

Reversibility:

not specified

Remarks on result:

no indication of irritation

Results 3

Irritation parameter:

iris score

Remarks:

For rinsed eye

Basis:

mean

Time point:

72 h

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Results 4

Irritation parameter:

cornea opacity score

Remarks:

For rinsed eye

Basis:

mean

Time point:

72 h

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

For rinsed eye : 0.33 for chemosis, 0.89 for redness of the conjunctiva, 0 for iris lesions and 0 for corneal opacity. Redness and chemosis were fully reversible within 10 and 3 days respectively.

Other effects:

not specified

Any other information on results incl. tables

Washing procedure did not change the ocular reactions.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

Based on the observation and under the test experimental condition, the test chemical was considered as non-irritant to the eye of treated rabbits.

Executive summary:

An acute ocular irritation study was conducted for the given test chemical to determine the potential to induce eye irritation in 9 rabbits according to methods similar to OPPTS 870.2400 and OECD 405 guidelines. During the study, a single dose of 100 mg of the test chemical was instilled into one eye, the other eye was not treated and served as control. For 6 rabbits, the eyes were not rinsed after administration of the test chemical while a washing procedure was applied for 3 other animals. Ocular reactions were observed 24, 48 and 72 hours after the administration and then daily until reversibility of the ocular reactions. The values of the scores for chemosis, redness of the conjunctiva, iris lesions and corneal opacity were noted for each animal. Mean scores calculated over 24, 48 and 72 hours for the 6 animals without rinsing were 0.33 for chemosis, 0.89 for redness of the conjunctiva, 0 for iris lesions and 0 for corneal opacity. Redness and chemosis were fully reversible within 10 and 3 days respectively. Washing procedure did not change the ocular reactions. Thus, under these experimental conditions, test chemical was considered as non-irritant when administered by ocular route to rabbits.