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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose:
read-across source
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
WoE report is based on toxicity study of microorganism for the test chemical :
1 and 2) Short term toxicity of test material on microorganism by disc diffusion method
GLP compliance:
not specified
Test organisms (species):
other: S. aureus, B. cereus , E. coli , P. aeruginosa
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Details on test conditions:
1 and 2)- Test vessel: Petri plate
-The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×108 1×106 CFU/ml for bacteria and fungi respectively. Inoculate was wabbed on Muller Hinton Agar.Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates.
Duration:
48 h
Dose descriptor:
other: MIC
Effect conc.:
> 8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
48 h
Dose descriptor:
other: MIC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Conclusions:
The test chemical is likely to be toxic to microorganism atleast in the concentartion range of range of 1 to 8 mg/l
Executive summary:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be in the range of 1 - 8 mg/l.Based on the above effect concentartion it can be considered that test substance is moderately toxic to microorganisms.

 

Description of key information

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be in the range of 1 - 8 mg/l.Based on the above effect concentartion it can be considered that test substance is moderately toxic to microorganisms.

 

Key value for chemical safety assessment

EC50 for microorganisms:
8 mg/L

Additional information

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be in the range of 1 - 8 mg/l.Based on the above effect concentartion it can be considered that test substance is moderately toxic to microorganisms.

1)Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be >8 mg/l.

2) Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method.The organisms used for the test wereS. aureus, B. cereus , E. coli , P. aeruginosa.The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×1081×106CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile

blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control.The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l