Registration Dossier
Registration Dossier
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EC number: 212-141-7 | CAS number: 765-14-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Freshwater
The following statistical effect concentrations were obtained in an acute algae growth inhibition test after 72 hours of exposure:
The Algal growth rate ErC10/50 and LOErC was determined to be > 100 mg/L, the NOErC was determined to be 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Key study
In the GLP and OECD 201 guideline compliant study the test substance was exposed to the Pseudokirchneriella subcapitata in the concentrations of 0 (control), 1, 3.2, 10, 32, 100 mg/L.
The water pH, temperature and lighting intensity were all maintained within acceptable guideline specifications. The following statistical effect concentrations were obtained after 72 hours of exposure:
The Algal yield EyC10/50 and LOEyC was determined to be > 100 mg/L, the NOEyC was determined to be 100 mg/L.
The Algal growth rate ErC10/50 and LOErC was determined to be > 100 mg/L, the NOErC was determined to be 100 mg/L.
The concentration of test substance in test media could not be verified analytically; therefore the effect concentrations are based on the loading rate used to prepare the test substance WAF solutions. According to guidance in OECD 23, for tests with chemicals that cannot be quantified by analytical methods at the concentrations causing effects, the effect concentration can be expressed based on the nominal concentrations or in the case of water accommodated fractions on the loading rate.
This test was conducted utilizing modified exposure media, closed test system, and reduced inoculation density. An additional reference toxicant test was conducted with the same method modifications described in this study to determine any effect on algal response to toxic stress. The results of a reference toxicant test indicate that the algae are responding normally to toxicant stress under the modified test conditions and that these deviations from test guidelines do not affect the results of this study.
The results in this study are consistent with all validity criteria and the test is valid according to the guidelines of this study.
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