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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017-06-05 to 2017-06-17
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
The test concentration and stability in test media were not analysed. The results were reported as nominal concentration only.
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
yes
Remarks:
Aceton
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 500mg of the test substance were solved in 0.5mL solvent and filled up to 1mL with the solvent. 10µL of the stock solution were added to the test medium (1200mL) to prepare the final test concentration.
- Controls: control and vehicle control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium: 100µL/L (10µL added to 100mL)
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain/clone: Daphnia magna
- Source: MicroBio Test Inc, Kleimoer 15, 9030 Mariakerke (Gent), Belgium
- Feeding during test: No.

ACCLIMATION
- Acclimation period: 48h
- Acclimation conditions (same as test or not): yes
- Type and amount of food: live algal cells (Pseudokirchneriella subcapitata)
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
201.6 mg/L as CaCO3
Test temperature:
20.1 - 20.8°C
pH:
7.56 - 7.86
Dissolved oxygen:
8.09 - 8.95 mg/L
Nominal and measured concentrations:
Control, vehicle control (Acetone) and 50.0 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker of 600 mL capacity filled with 100 mL of test media
- Aeration:
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was used as the test media. It was prepared by adding different chemicals to distilled water.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h dark cycle
- Light intensity: 1330 and 1340 Lux

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: control, vehicle control, 0.01, 0.1, 1.0, 10.0, 25.0 and 50.0 mg/L.
- Results used to determine the conditions for the definitive study: no immobility observed
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities:
- Other biological observations: Daphnids exposed to 50.0 mg OEA/L, control (without OEA) and vehicle control (acetone) did not exhibit any abnormal behavioural symptoms at 24 and 48 h.
- Mortality of control: none
Results with reference substance (positive control):
- Results with reference substance valid? yes
-The highest tested concentration of potassium dichromate causing no immobilisation and the No Observed Effect Concentration (NOEC) was 0.09 mg/L and the lowest tested concentration causing 100% immobilisation within the 48 h test period was 2.11 mg/L. The Lowest Observed Effect Concentration (LOEC) was 0.20 mg/L over the 48 h exposure period of potassium dichromate. The 24 and 48 h EC50 of potassium dichromate were determined 1.06 and 0.26 mg/L, respectively.

Table 1: Immobility Data at 0, 24 and 48 h Exposure Period

Group

Test Concentration

(mg/L)

N° of Replicates

N° of Daphnia/group

Immobility N° and % at

0 h

24 h

%

48 h

%

G1

0.0 (Control)

4

20

0

0

0

0

0

G2

0.0 (Vehicle Control)

4

20

0

0

0

0

0

G3

50.0

4

20

0

0

0

0

0

Table 2: Mean Values of Water Parameters of Test Solution

Groups

Test

Concen-tration

 (mg/L)

pH

Temperature (°C)

Dissolved Oxygen (mg/L)

Total Hardness

(as CaCO3)

mg/L water

0 h

48 h

0 h

48 h

0 h

48 h

Mean

SD

Mean

SD

Mean

SD

Mean

SD

Mean

SD

Mean

SD

201.6

G1

0.0 (Control)

7.63

0.017

7.77

0.054

20.8

0.06

20.5

0.08

8.77

0.132

8.32

0.108

G2

0.0 (Vehicle Control)

7.57

0.008

7.79

0.030

20.6

0.10

20.3

0.17

8.74

0.015

8.26

0.180

G3

50.0

7.56

0.005

7.80

0.046

20.4

0.10

20.6

0.13

8.80

0.024

8.26

0.116

Validity criteria fulfilled:
yes
Conclusions:
The toxicity of the test item OEA was examined in a acute daphnia test according to OECD 202 and under GLP conditions. Individuals of D. magna were exposed to a control, a vehicle control and 50 mg/L of the test substance for 48 hours. As no immobility was observed in the highest test concentration, the 48-hour EC50 was determined as > 50 mg/L based on nominal concentration.

Description of key information

EC50 (48-h) > 50 mg/L based on nominal concentration

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
50 mg/L

Additional information

The toxicity of the test item OEA was examined in an acute daphnia test according to OECD 202 and under GLP conditions. Individuals of D. magna were exposed to a control, a vehicle control and 50 mg/L of the test substance for 48 hours. As no immobility was observed in the highest test concentration, the 48-hour EC50 was determined as > 50 mg/L based on nominal concentration.