Reaction mass of [5-methyl-2-[[4-(4-methylanilino)-9,10-dioxo-1-anthryl]amino]phenyl]sulfonyloxypotassium and [5-methyl-2-[[4-(4-methyl-2-potassiooxysulfonyl-anilino)-9,10-dioxo-1-anthryl]amino]phenyl]sulfonyloxypotassium

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 17 July to 2 November, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

adopted in 1995

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Water solubility:

15.94 g/L

Conc. based on:

test mat.

Incubation duration:

24 - 72 h

Temp.:

20 °C

pH:

5.91 - 6.13

Remarks on result:

other: average

Details on results:

To verify the accuracy of the analytical method a fresh prepared standard solution (prepared with the test item) was measured in between the samples of the flask method.
The recovery rate of the measured standard solutions was in the range from 100.1 % to 100.9 %, which shows a good accuracy of the analytical method.

PRELIMINATY TEST
The preliminary test at room temperature showed, that the water solubility of the test item was between 1.02 g/l and 7.5 g/l.

Results of flask method

Flask	1a	2a	3a	4a
measured value [mg/l]	306.7 / 304.2	322.0 / 321.8	329.4 / 329.2	-- / --
average water solubility [g/l]	15.27	16.1	16.47	--
mean value of the water solubility [g/l]	15.94			--
standard deviation [g/l]	0.61			--
relative standard deviation [%]	3.84			--
pH conditions	5.91	6.06	6.13	6.69

Measurement of a standard solution

Standard solution (nominal value [g/l])	Measured value [g/l]	Repeatability [%]
Ü-1 (0.3298)	0.3304	100.2
	0.3307	100.3
	0.3302	100.1
	0.3300	100.1
Ü-2 (0.4308)	0.4348	100.9
	0.4327	100.4
	0.4334	100.6
	0.4330	100.5

Conclusions:

Water solubility: 15.94 g/l at 20 °C and pH 5.91-6.13

Executive summary:

Water solubility test was tested, according to the OECD guideline 105; flask method was used. A preliminary test was carried out to determine the suitable method to be used in the main test. In the preliminary test the solubility of the test item was between 1.02 g/l and 7.5 g/l. Therefore, the flask method was used in the main test.

Conclusion

Water solubility: 15.94 g/l at 20 °C and pH 5.91-6.13

Description of key information

Water solubility: 15.94 g/l at 20 °C and pH 5.91-6.13

Key value for chemical safety assessment

Water solubility:

15.94 g/L

at the temperature of:

20 °C

Additional information

OECD 105 - Flask method