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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003-06-23 to 2003-10-01
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study performed according to OECD 305 and METI Guideline: Concentration Test on Chemical Substances in Fish.
Qualifier:
according to guideline
Guideline:
other: OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Qualifier:
according to guideline
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Puritiy: 99.78 % (GC)
Analytical monitoring:
yes
Details on sampling:
- Sampling intervals/frequency for test medium samples: days 0, 4, 7, 11, 14, 18, 21, 25, 28, 39, 32, 35, 39, 42
Vehicle:
yes
Details on test solutions:
According to guideline

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 50 mg/l

Dose 1: Test item: 0.1 mg/L ,
Dose 2: Test item: 0.01 mg/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Cyprinus carpio
Date: 10. Jul 2003
Lot: 030107c
Weight: 7.8 g
Length: 8.7 cm

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
42 d
Hardness:
250 g/L
Test temperature:
25.0 ° +/- 0.4 °C
pH:
7
Dissolved oxygen:
saturated
Nominal and measured concentrations:
nominal concentration 0.10 mg/L:
Solubilizer used: HCO-40, 50 mg/L

analytically measured concentrations:
day 4: 0.10100 mg/L
day 7: 0.0992 mg/L;
day 11: 0.100 mg/L
day 14: 0.100 mg/L
day 18: 0.0982 mg/L
day 21: 0.0982 mg/L
day 25: 0.100 mg/L
day 28: 0.100 mg/L
day 32: 0.0988 mg/L
day 35: 0.100 mg/L
day 39: 0.100 mg/L
day 42: 0.0992 mg/L

nominal concentration 0.01 mg/L
Solubilizer used: HCO-40, 5 mg/L
analytically measured concentrations:
day 4: 0.00996 mg/L
day 7: 0.00996 mg/L;
day 11: 0.0100 mg/L
day 14: 0.0101 mg/L
day 18: 0.0101 mg/L
day 21: 0.0100 mg/L
day 25: 0.00996 mg/L
day 28: 0.0101 mg/L
day 32: 0.00982 mg/L
day 35: 0.0102 mg/L
day 39: 0.0100 mg/L
day 42: 0.0101 mg/L

Details on test conditions:
After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 0, 5, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.2 and 0.02 mg/L were maintained during the experimental phase.


Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Concentrations maintained using HCO-40
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Concentrations maintained using HCO-40
Duration:
42 d
Dose descriptor:
NOEC
Effect conc.:
0.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Concentrations maintained using HCO-40
Validity criteria fulfilled:
yes
Conclusions:
The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.1 mg/L and thus higher than the limit of water solubility.
Executive summary:

The purpose of this study was to determine the bioconcentration potential of the test material in fish under flow-through conditions. During the GLP compliant test the lethal and other effects in fish were determined during the exposure period of 42 days.

The test material showed no toxic potential at concentrations at or above the water solubility. The NOEC was 0.1 mg/L and thus higher than the limit of water solubility.

Description of key information

Data from a GLP study according to OECD TG 305 are available.

NOEC (28 d) = 0.10 mg/L (50 mg/L HCO-40)

LC50 (28 d)  > 0.10 mg/L (50 mg/L HCO-40)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.1 mg/L

Additional information

NOEC (BCF, 28d) > 0.10 mg/L