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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04 Dec 2000 - 08 Dec 2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No European GLP
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Deviations:
no
GLP compliance:
yes
Test material information:
Composition 1
Specific details on test material used for the study:
Batch: E004115
Analytical monitoring:
yes
Details on sampling:
Samples were taken (in duplicate) during the Limit Test at 0, 24, 48, 72 and 96 h.
Vehicle:
yes
Details on test solutions:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
The test solution was prepared by directly adding appropriate amounts in dilution water and then facilitating its dispersion by ultrasonification for 30 min.

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Solutions prepared with vehicle
- Controls: vehicle control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 1000 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name:Oryzias latipes
- Strain: Oryzias latipes
- Source: Commercial Supplier, Japan
- Length at study initiation: 2.9 cm (average length)
- Weight at study initiation: 0.21 g (average weight)
- Feeding during test: commercial fish diet during acclimatization

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): no
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no
Test temperature:
24.4 °C
pH:
8
Dissolved oxygen:
88% - 91%
Salinity:
no data
Nominal and measured concentrations:
Nominal Concentration: 250 mg/L
Mean measured concentration: 250 mg/L
Details on test conditions:
TEST SYSTEM
- Type: open
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking water
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: light dark cycle was 16h:8h


TEST CONCENTRATIONS
- Range finding study: Yes
- Test concentrations: 250 mg/L
Reference substance (positive control):
no
Remarks:
The test with the reference substance is performed at least once each batch of fish as a means of assuring that the lab test conditions are adequate and have not changed significantly.
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 250 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes
Validity criteria fulfilled:
yes
Conclusions:
No mortality was observed in this assay up to the limit concentration of 250 mg/L. The concentration was maintained using HCO as solubiliser. The LC50 (96 h) exceeds 250 mg/L.
Executive summary:

The test was performed according to OECD 203. No mortality was observed in this assay up to the limit concentration of 250 mg/L. The concentration was maintained using HCO as solubiliser. The LC50 (96 h) exceeds 250 mg/L.

Description of key information

The results showed that under valid static test conditions, the 96 h-LC50 of the test material to fish (Oryzias latipes) is greater than the nominal concentration of 250 mg/L. Thus, the 96h-LC50 is greater than 250 mg/L.

Key value for chemical safety assessment

Additional information