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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29 Nov 2000 - 05 Mar 2001
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
Batch: E004115

Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 4, 11, 25, 32, 46, 60
- Sampling intervals/frequency for test medium samples: days 4, 11, 25, 32, 46, 60
- Fish: two fish per sampling homogenised, and extracted with THF. Further analysis performed by GC-MS.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
HCO-40, 800 mg/LPREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Solutions prepared with vehicle
- Controls: vehicle control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 800 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Length: 7.3 - 10.0 cm
Batch: TFC-000920-II
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
60 d
Test temperature:
25 +/- 2 °C
pH:
7.7-8.1
Dissolved oxygen:
7.6 - 8.1 mg/L
Details on test conditions:
according to guidelne
Nominal and measured concentrations:
Nominal concentration: 0.1 mg/L
Measured on day
4 0.0951 mg/L
11 0.1000 mg/L
25 0.0981 mg/L
32 0.0962 mg/L
46 0.0961 mg/L
60 0.0979 mg/L

Nominal concentration: 0.01 mg/L
Measured on day
4 0.00930 mg/L
11 0.00977 mg/L
25 0.00927 mg/L
32 0.00956 mg/L
46 0.00992 mg/L
60 0.00968 mg/L
Key result
Conc. / dose:
0.1 mg/L
Temp.:
20 °C
pH:
7.9
Type:
BCF
Value:
45 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
24 d
Key result
Conc. / dose:
0.01 mg/L
Temp.:
25 °C
pH:
7.9
Type:
BCF
Value:
150 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
40 d
Remarks on result:
other: Conc.in environment / dose:0.002 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The mean BCF for the test material as determined in a flow through test system amounts to 82 in this test system.

Description of key information

This study was performed according to GLP and the methods applied are fully compliant with OECD TG 305.

Key value for chemical safety assessment

BCF (aquatic species):
82 dimensionless

Additional information

Bioaccumulation was tested in fish (Cyprinus carpio) exposed for 60 days to the test material at concentrations of 0.1 and 0.01 mg/L in a flow through system. Regular concentration control analysis of the test media showed that the test material concentrations were maintained at about 95 % of the nominal values, throughout the exposure period and that the test material was present in dissolved form, thus proving steady state exposure conditions. No signs of toxicity were observed throughout the study. Steady state BCFs of 150 and 45 (mean 82) were determined. Due to the low bioaccumulation potential, no depuration phase was required. Based on these data it is concluded that the test material showed no relevant bioaccumulation (BCF < 2000).