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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Mar 22, 2000 - May 30, 2000
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
Batch: N010065141
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant
- OriginMunicipal sewage treatment plant
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice.
- Concentration of suspended solids per litre: 30 mg
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
According to guideline
Reference substance:
aniline
Preliminary study:
The biodegradation in % was calculated based on the ThOD of 2.540 mg 02/mg test item.
Test performance:
The reference item (Aniline) was sufficiently degraded to 71 % after 14 days and to 77% after 28 days of incubation.The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Key result
Parameter:
% degradation (test mat. analysis)
Value:
0
Sampling time:
28 d
Results with reference substance:
14d: 71 %, 28d: 77 %
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Executive summary:

The informtion for this endpoint study record was obtained from an experimental study. The OECD GLP criteria were met and the methods applied are fully compliant with OECD TG 301C. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.

Description of key information

The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation in a study according to OECD 301C. Thus, the test item is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed
Type of water:
freshwater

Additional information