1-Ethoxy-2,3-difluoro-4-(trans-4-propylcyclohexyl)-benzene

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09/05/2007-12/08/2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain/clone: Daphnia Magna
- Source: in house
- Age of parental stock: younger than 24 hours
- Feeding during test: no
ACCLIMATION
- Acclimation period: 5 days
- Acclimation conditions: same as test (controlled environment room, pH 7.9+/-0.3)
- Type and amount of food: green algea (Scenedesmus subspicatus)
- Health during acclimation: no mortality observed
At the start of the experimental phase 5 Daphnia magna were placed in each test vessel containing 10 mL reconstituted water with or without test material. The test vessels were left open.

Test type:

other: open-static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

14o dH (about 250 mg/L CaCO3)

Test temperature:

18 – 22 °C

pH:

7.9 +/- 0.3

Dissolved oxygen:

at least 90 %

Nominal and measured concentrations:

Nominal 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open static
- Material: test item in reconstituted water
- Aeration: 24 hours after preparation
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: -
-TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted according to ELENDT (1990)
- Macro nutrients: mg/L
CaCl2 x 2H2O: 293.80
MgSO4 x 7H2: 123.30
NaHCO3: 64.80
KCl: 5.80
Na2SiO3 x 9H2O: 10.00
NaNO: 30.27
KH2PO: 40.14
K2HPO: 40.18
- Trace elements: mg/L
B: 0.5000
Fe: 0.2000
Mn: 0.1000
Li, Rb and Sr: 0.0500
Mo: 0.0250
Br: 0.0125
Cu and Zn: 0.0063
Co and I: 0.0025
Se: 0.0010
V: 0.0003
- Macro nutrients
Na2EDTA x 2H2O: 2.50
- Vitamins µg/L
Thiamine: 75.00
B12: 1.00
Biotin: 0.75
- Total organic carbon: --
- Particulate matter: --
- Metals: --
- Pesticides: --
- Chlorine: --
- Alkalinity: --
- Ca/mg ratio: --
- Conductivity: --
- Culture medium different from test medium: no
- Intervals of water quality measurement: 48 h
OTHER TEST CONDITIONS
- Adjustment of pH: 7.9
- Photoperiod: 16h light / 8h dark
- Light intensity: --

References: ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.Protoplasma 154, 25-33, 1990

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

In this open static test system, Daphnia magna exposed to a nominal concentration of 100 mg/L revealed no biological effects.

Results with reference substance (positive control):

Potassium dichromate: 24 h EC50 0.95 (0.87-1.04) mg/L; 48 h EC50 0.74 (0.68-0.81) mg/L

Validity criteria fulfilled:

yes

Conclusions:

The test material showed no aquatic toxicity, thus the 48 hours NOEC and EC50 to Daphnia magna for the test item are > 100 mg/L (nominal).

Executive summary:

Purpose

The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.

 

Study design

For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The GLP study was performed as a limit test according to OECD 202.

 

Results

The following results have been obtained:

   

Nominal concentration Immobilization immobilized / exposed
[g/L]	24 hours	48 hours
0.0	0/20	0/20
0.1	0/20	0/20

 

Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L of test item were not affected.

 

For the test material the following EC50 values for Daphnia magna were determined:

24 h EC50 > 0.1 g/L (nominal > 100 mg/L)

48 h EC50 > 0.1 g/L (nominal > 100 mg/L)

NOEC > 0.1 g/L (nominal)

LOEC > 0.1 g/L (nominal)

 

Conclusions

Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC and EC50 to Daphnia magna are > 100 mg/L (nominal).

Description of key information

The test material showed no aquatic toxicity, thus the 48 hours NOEC and EC50 to Daphnia magna for the test item are > 100 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L

Additional information

Purpose

The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments.

 

Study design

For this purpose, juvenile Daphnia magna were exposed to an aqueous test material preparation over 48 hours, under defined conditions. The GLP study was performed as a limit test according to OECD 202.

 

Results

The following results have been obtained:

   

Nominal concentration Immobilization immobilized / exposed
[g/L]	24 hours	48 hours
0.0	0/20	0/20
0.1	0/20	0/20

 

Daphnia magna exposed to an aqueous preparation of the nominal concentration of 100 mg/L of test item were not affected.

 

For the test material the following EC50 values for Daphnia magna were determined:

24 h EC50 > 0.1 g/L (nominal > 100 mg/L)

48 h EC50 > 0.1 g/L (nominal > 100 mg/L)

NOEC > 0.1 g/L (nominal)

LOEC > 0.1 g/L (nominal)

 

Conclusions

Under the given experimental conditions, the test material showed no aquatic toxicity. The 48 hours NOEC and EC50 to Daphnia magna are > 100 mg/L (nominal).