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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From October 5 to 7, 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
3,3'-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]imino]bis[propiononitrile]
EC Number:
223-765-4
EC Name:
3,3'-[[4-[(2-chloro-4-nitrophenyl)azo]phenyl]imino]bis[propiononitrile]
Cas Number:
4058-30-4
Molecular formula:
C18H15ClN6O2
IUPAC Name:
3,3'-({4-[(2-chloro-4-nitrophenyl)diazenyl]phenyl}imino)dipropanenitrile
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0.058 - 0.10- 0.18 - 0.32 - 0.58 - 1.0 and 1.8 mg test article /l nominal.
- Stock solution: 0.1 g Test substance were mixed with 200 ml test medium and fill up to 1000 ml with test medium. The stock solution was homogenized for five minutes in the supersonics bath.
- Sample storage conditions before analysis: all test medium samples for the analytical determinations were taken after mixing of the test solutions from the approximate center of the test vessels and kept at -18°C to -25 °C until analyses.

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: Textile Dyes Division, Product Ecology TF
- Age at study initiation: animals with age of less than 24 hours were used

OTHER INFROMATION
The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
136mg/l CaCO3
Test temperature:
21 ± 1°C
pH:
in a range of 7.8 - 8.2
Dissolved oxygen:
oxygen saturated at the beginning of the test
Salinity:
-
Nominal and measured concentrations:
Nominal concentration: 0.058 - 0.10 - 0.18 - 0.32 - 0.58 - 1.0 and 1.8 mg test article /l
Measured concentration: in the range of 89.0 - 137.9 % of the nominal values.
Details on test conditions:
TEST SYSTEM
The daphnids were exposed to the test article in 250 ml beakers containing 100 - 200 ml test medium with 10 animals per beaker.
All tests are running in duplicate.
During exposure the animals were kept at 21 ± 1°C with 16 hours illumination.

TEST MEDIUM / WATER PARAMETERS
Stock solution Concentration in test
NaHCO3 65 mg/l
MgSO4 * 7 H2O 123.3 mg/l
KCl 5.8 mg/l
CaCI2 * 2 H2O 294 mg/l
Na2SiO3*9H2O 10 mg/l
NaNO3 274 µg/l
KH2PO4 143 µg/l
K2HPO4 184 µg/l

The test water medium was prepared with bidistilled water.
Theoretical water hardness: 136mg/l CaCO3
Ca / Mg ratio: 4 : 1
Na / K ratio: 10 : 1
Alkalinity: 0.8 mmol/l
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.08 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Oxygen concentration and pH

The oxygen concentration and the pH was controlled at the beginning and at the end of the test.

Start: Oxygen - saturated

Ph - 8.0 (stock solution)

Temperature –21.5 °C

 

End:

Nominal conc (mg/l)

Blank

0.058

0.10

0.18

0.32

0.58

1.0

1.8

Oxygen (%)

99

97

97

97

97

99

99

98

pH

8.2

8.1

8.1

8.1

8.1

8.1

8.1

8.1

Temperature (°C)

21.5

21.5

21.5

21.5

21.5

21.5

21.5

21.5

 

Calculation of EC - values

The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 89.0 -137.9 % of the nominal values. Therefore under the test conditions the test article was sufficiently stable. In all test concentrations none alteration of the test substance after 48 hours was observed. In the control and in the test concentrations up to 0.34 mg/l no immobilization of the daphnids was observed after 48 hours. The EC0 value is least 0.34 mg/l. The EC100 value is close to 1.78 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48-h EC50 to Daphnia magna was determined at 1.08 mg/L.
Executive summary:

Method

The acute toxicity of the test substance toDaphnia magnawas determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2.The study was performed under Good Laboratory Practice (GLP) conditions in Switzerland.

Observations

The nominal concentrations tested were 0.058 - 0.10 - 0.18 - 0.32 - 0.58 - 1.0 and 1.8 mg test article /l , and a control without any additions. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 89.0-137.9 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations none alteration of the test substance after 48 hours was observed. In the control and in the test concentrations up to 0.34 mg/l no immobilization of the daphnids was observed after 48 hours. The EC0 value is at least 0.34 mg/l. The EC100 values is close to 1.78 mg/l

 

Conclusion

EC0 = 0.34 mg/l

EC50 = 1.08 mg/l

EC100 > 1.78 mg/l