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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Bioaccumulation: aquatic / sediment

BCFBAF model (v3.01) of Estimation Programs Interface (EPI Suite, 2017) was used to predict the bioconcentration factor (BCF) of test chemical 4 -hydroxy-6 -(methylamino) naphthalene-2 -sulfonic acid (CAS No. 6259 -53 -6). The bioconcentration factor (BCF) of 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated to be 3.162 L/kg whole body w.w (at 25 deg C) which does not exceed the bio concentration threshold of 2000, indicating that the chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid is not expected to bioaccumulate in the food chain.

Additional information

Bioaccumulation: aquatic / sediment

Various predicted data for the target compound 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid (CAS No. 6259-53-6) and supporting weight of evidence study for its structurally similar read across substance were reviewed for the bioaccumulation end point which are summarized as below:

 

In aprediction done using theBCFBAF Program(v3.01) of Estimation Programs Interface (EPI Suite, 2017) was used to predict the bioconcentration factor (BCF) of test chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid (CAS No. 6259 -53 -6). The bioconcentration factor (BCF) of 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated to be 3.162 L/kg whole body w.w (at 25 deg C).

 

In an another prediction done by using Bio-concentration Factor (v12.1.0.50374) moduleACD (Advanced Chemistry Development)/I-Lab predictive module, 2017), theBio-concentration Factor (BCF) over the entire pH scale (pH 1 -14) of the test substance 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid (CAS no. 6259 -53 -6) was estimated to be 1.

 

Bioconcentration Factor (BCF) of test chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated using Chemspider database(ChemSpider, 2017). The bioconcentration factor of test substance 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated to be 1 at both pH 5.5 and 7.4, respectively.

 

Another predicted data was estimated usingSciFinder database (American Chemical Society (ACS), 2017) was used for predicting the bioconcentration factor (BCF) of test chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid (CAS No. 6259 -53 -6).The bioconcentration factor (BCF) of 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated to be 1 (at 25 deg C).

 

From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical), the bioaccumulation i.e BCF for test substance 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid was estimated to be 4.89 dimensionless . The predicted BCF result based on the 5 OECD principles. Thus based on the result it is concluded that the test substance 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid is non-bioaccumulative in nature.

 

In a supporting weight of evidence study from authoritative database (J-CHECK, 2017) for the read across chemical 1,3-Naphthalenedisulfonic acid, 7-hydroxy-8-((4-sulfo-1-naphthalenyl)azo)-, trisodium salt (CAS no. 2611-82-7),bioaccumulation experiment was conducted on test organism Cyprinus carpio for 28 days for evaluating the bioconcentration factor (BCF value) of read across chemical 1,3-Naphthalenedisulfonic acid, 7-hydroxy-8-((4-sulfo-1-naphthalenyl)azo)-, trisodium salt. The study was performed according to other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances". Cyprinus carpio was used as a test organism for the study. Details on analytical methods involve the recovery ratio: Test water: 100% (The test solution were input analytical equipment directly), Fish: 82.4%, - Limit of quantitation: Test water: 1st concentration area : 21µg/l, 2nd concentration area : 2.1µg/l, Fish: 260 ng/g. Test chemical nominal conc. used for the study were 0.474mg/land 0.0474 mg/l, respectively. Range finding study involve the LC50 (96 hr) > 200 mg/l onRice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance 1,3-Naphthalenedisulfonic acid, 7-hydroxy-8-((4-sulfo-1-naphthalenyl)azo)-, trisodium salt on Cyprinus carpio was determined to be ≤ 0.55 L/Kg at a conc. of 0.474 mg/l and ≤ 5.6 L/Kg at a conc. of 0.0474 mg/l, respectively.

 

Another bioaccumulation study was conducted for estimating the BCF (bioaccumulation factor) value of read across chemical 1,3 -Naphthalenedisulfonic acid, 7 -hydroxy-8 -((4 -sulfo-1 -naphthalenyl)azo)-, trisodium salt (CAS no. 2611 -82 -7) (HSDB, 2017). The bioaccumulation factor (BCF) value was calculated using a measured water solubility of 80,000 mg/l and a recommended regression-derived equation. The estimated BCF (bioaccumulation factor) value of 1,3 -Naphthalenedisulfonic acid, 7 -hydroxy-8 -((4 -sulfo-1 -naphthalenyl)azo)-, trisodium salt was determined to be 1.0 dimensionless.

 

For the read across chemical calcium 3-hydroxy-4-[(4-methyl-2-sulfonatophenyl)diazenyl]-2-naphthoate (CAS no. 5281-04-9) from authoritative database (J-CHECK, 2017) and secondary source (OECD SIDS, 1994),bioaccumulation experiment was conducted on test organism Cyprinus carpiofor 6 weeks for evaluating the bioconcentration factor (BCF value) of read across chemical calcium 3 -hydroxy-4 -[(4 -methyl-2 -sulfonatophenyl)diazenyl]-2 -naphthoate. The study was performed according to OECD Guideline 305 (Bioconcentration: Flow-through Fish Test) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively at a temperature of 25°C. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 0.3mg/land 0.03 mg/l, respectively. Test chemical solution was prepared in HCO-40 and Crystal sugar. Analytical method involve therecovery ratio: Fish : 79.6 %, - Limit of quantitation : Test water : 1st concentration area : 0.017 microg/mL, 2nd concentration area : 0.00 microg/mL, Fish : 0.18 microg/g.Range finding study involve theLC50(48h) >= 50.0 mg/LonRice fish (Oryzias latipes).The bioconcentration factor (BCF value) of substance calcium 3-hydroxy-4-[(4-methyl-2-sulfonatophenyl)diazenyl]-2-naphthoateonCyprinus carpio was determined to be in the range of ≤ 0.7-1.8 L/Kg at a conc. of 0.3 mg/l and ≤ 6.9 L/Kg at a conc. of 0.03 mg/l, respectively.

 

On the basis of above results for target chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid (from EPI suite, ACD labs,ChemSpider, Scifinder and CompTox Chemistry Dashboard,  2017) and for its read across substance from authoritative database (HSDB & J-CHECK) and (secondary source OECD SIDS), it can be concluded that the BCF value of test substance 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid ranges from 1 – 4.89 which does not exceed the bioconcentration threshold of 2000, indicating that the chemical 4-hydroxy-6-(methylamino)naphthalene-2-sulfonic acid is not expected to bioaccumulate in the food chain.