14H-benz[4,5]isoquino[2,1-a]perimidin-14-one

Particle size distribution (Granulometry)

Link to relevant study record(s)

Reference

Endpoint:

particle size distribution (granulometry)

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 110 (Particle Size Distribution / Fibre Length and Diameter Distributions) - Method B: Fibre Length and Diameter Distributions

Deviations:

no

Principles of method if other than guideline:

In consideration of the relevance of granulometry study on toxicological assessment, only the size distributions of the sieved fraction < 100 µm were studied in detail.

GLP compliance:

no

Other quality assurance:

ISO/IEC 17025 (General requirements for the competence of testing and calibration laboratories)

Type of method:

microscopic examination

Type of distribution:

counted distribution

Mass median aerodynamic diameter:

9.07 µm

Percentile:

D50

Mean:

9.07 µm

Remarks on result:

other: Migrated from fields under 'Mass median diameter' as D50 percentile. No source field for Standard deviation.

The sample was first sieved by a 100 µm sieve.

weighted sample: 0.5847 g

weight fraction < 100 µm: 0.0066 g

mass fraction < 100 µm: 1.13 %

The mass fraction < 100 µm was 1.13 %, and was investigated by scanning eletron microscopy. The median acpect ration was 2.00 with 0.93 as standard deviation.

The median diameter of the particle size is given for the fraction < 100 µm. The value of 9.07 µm was calculated for spherical particle and a value of 8.19 µm was calculated for cubical particle.

Particle size	Spherical Volume [%]	Cubical Volume [%]
< 4 µm (respirable)	0.40	0.88
4 - 10 µm (thoracic)	11.33	21.11
10 - 100 µm (inhalable)	88.27	78.01
> 100 µm	0.00	0.00
Median (diameter µm)	9.07	8.18

Conclusions:

The MMAD (Mass Median Aerodynamic Diameter < 100 μm) has a value of 9.07 μm as spherical particles.

Executive summary:

According to OECD guideline 110, the particle size distribution of 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one was determined by scanning microscopy method for the sieved fraction (< 100 µm, 1.13 % of total sample) and presented on the basis of calculated mass fractions. The median diameter was 9.07 µm with the mean fraction of 88.27 % distribution in the range of 10 to 100 µm; and hence the tested 14H-benz[4,5]isoquino[2,1-a]perimidin-14-one particles with sieved size smaller than 100 µm are mainly dominated by inhalable fraction as defined in EN 481.

Description of key information

The MMAD (Mass Median Aerodynamic Diameter < 100 μm) has a value of 9.07 μm as spherical particles.