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Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005-06-28 - 2005-07-26
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Non-adapted activated sludge was collected from the sewage plant at Hildesheim (Germany) which treats mostly municipal sewage (industrial chemical waste: ca. 30%, referring to BOD) and used as source of the inoculum
- Laboratory culture: no
- Preparation of inoculum for exposure: The activated sludge was filtered with folded filter. The first 200 mL of the filtrate were discarded. The second filtrate effluent from the domestic waste water was used to initiate inoculation.
- Pretreatment: no
- Concentration of sludge: <=30 mg/L suspended solids
- Initial cell/biomass concentration: 10E4 to 10E6 CFU/L
- Water filtered: not reported
Duration of test (contact time):
28 d
Initial conc.:
70 mg/L
Based on:
test mat.
Initial conc.:
89.6 other: mg O2/L
Based on:
COD
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: mineral nutrient solution acc. to OECD Guideline 301 F
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 20-21°C (temperature was documented by a thermohygrograph)
- pH: at the beginning: 7.68; at the end: 7.56-7.57 (pH was determined at the beginning and at the end of the study period after 28 d
- pH adjusted: no
- Aeration of dilution water: not reported
- Suspended solids concentration: <=30 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: brown glass bottles (incubator)
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: not reported
- Measuring equipment: OxiTop OC110 controller, WTW, OxiTop measuring heads, WTW, Incubator, RUBARTH, pH-Meter 340i, WTW; thermohygrograph
- Test performed in closed vessels: yes

SAMPLING
- Sampling frequency: oxygen consumption was determined continuously from the pressure in the incubation vessels by OxiTOP measuring system at 360 measuring points (every 112 minutes) during the 28 d incubation period.
- Sampling method: oxygen consumption was determined continuously from the pressure in the incubation vessels
- Sterility check if applicable: not reported
- Sample storage before analysis: no


CONTROL AND BLANK SYSTEM
- Inoculum blank: performed
- Abiotic sterile control: not reported
- Toxicity control: performed
- Other: functional/procedural control (reference substance) performed
Reference substance:
acetic acid, sodium salt
Test performance:
The ready biodegradability of Amphopropionates C12-18 was investigated in a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test).
Parameter:
% degradation (O2 consumption)
Value:
60
Sampling time:
12 d
Remarks on result:
other: the test item proved to be readily biodegradable and fulfilling the 10-d window criterion
Parameter:
% degradation (O2 consumption)
Value:
71
Sampling time:
28 d
Remarks on result:
other: the test item proved to be readily biodegradable and fulfilling the 10-d window criterion
Results with reference substance:
The reference substance sodium acetate was biodegraded by >60% after 2 d of incubation (for details see below). The validity criterion >60% biodegradation after 14 days was fulfilled.

In a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) the following results were obtained

Biodegradation (%) of Amphopropionates C12 -18, functional control (sodium acetate), and the toxicity control (test item + functional control)

Study day

Biodegradation (%)

Functional control

Test item

Toxicity control

R1

R2

P1

P2

T1

T2

0

0

0

0

0

0

0

2

100

100

3

13

51

53

5

100

100

52

49

67

75

8

100

100

55

55

77

83

12

100

100

60

60

97

100

15

100

100

61

68

100

100

20

100

100

53

56

99

100

25

100

100

71

68

100

100

28

100

100

72

69

100

100

The reference substance sodium acetate reached the pass-level (biodegradation>60%) already after 2 d. According to the results obtained in the toxicity control Amphopropionates C12 -18 are not inhibitory to the inoculum in the concentration used in the test (100% biodegradation after 14 d). The test item (test concentration: 70 mg/L corresponding to COD=89.6 mg O2/L) proved to be readily biodegradable and fulfilling the 10-d window criterion (71% biodegradation after 28 d; the 10% level was reached after 3 d and the 60% pass level after 12 d).

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
In a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) Amphopropionates C12-18 proved to be readily biodegradable and fulfilling the 10-d window criterion (71% biodegradation after 28 d; the 10% level was reached after 3 d and the 60% pass level after 12 d). As could be shown in the toxicity control, the test item at the test concentration applied (70 mg/L) was not inhibitory to the inoculum.
Executive summary:

In a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) the following results were obtained:

Biodegradation (%) of Amphopropionates C12 -18 40% in water, functional control (sodium acetate), and the toxicity control (test item + functional control)

Study day

Biodegradation (%)

Functional control

Test item

Toxicity control

R1

R2

P1

P2

T1

T2

0

0

0

0

0

0

0

2

100

100

3

13

51

53

5

100

100

52

49

67

75

8

100

100

55

55

77

83

12

100

100

60

60

97

100

15

100

100

61

68

100

100

20

100

100

53

56

99

100

25

100

100

71

68

100

100

28

100

100

72

69

100

100

The reference substance sodium acetate reached the pass-level (biodegradation>60%) already after 2 d. According to the results obtained in the toxicity control Amphopropionates C12 -18 40% in water are not inhibitory to the inoculum in the concentration used in the test (100% biodegradation after 14 d). The test item (test concentration: 70 mg/L corresponding to COD=89.6 mg O2/L) proved to be readily biodegradable and fulfilling the 10-d window criterion (71% biodegradation after 28 d; the 10% level was reached after 3 d and the 60% pass level after 12 d).

Description of key information

readily biodegradable (OECD TG 301 F; RL1; GLP): 71% biodegradation after 28 d; the 10% level was reached after 3 d and the 60% pass level after 12 d

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

In a study conducted according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) the following results were obtained:

The reference substance sodium acetate reached the pass-level (biodegradation>60%) already after 2 d. According to the results obtained in the toxicity control Amphopropionates C12 -18 (40% a.i.) is not inhibitory to the inoculum in the concentration used in the test (100% biodegradation after 14 d). The test item (test concentration: 70 mg/L corresponding to COD=89.6 mg O2/L) proved to be readily biodegradable and fulfilling the 10-d window criterion (71% biodegradation after 28 d; the 10% level was reached after 3 d and the 60% pass level after 12 d).

 

Supporting data are available from the following studies:

The ready biodegradability of Amphopropionates C12 -18 40% in water was investigated in a study conducted according to OECD Guideline 301 A (new version) (Ready Biodegradability: DOC Die Away Test).

The reference substance sodium acetate was biodegraded by 95% after 14 d. The validity criterion (>70% biodegradation after 14 days) was fulfilled.

The test item reached 61% degradation after 28 d and thus, was not readily biodegradable as the pass level of 70% was not reached within the test period. The biodegradation of the reference substance was not inhibited by the test item at the concentration tested (73% degradation after 28 d).

 

Amphopropionates C12 -18 proved to be ready biodegradable in the test performed according to the German DIN 38412 T.25 (Static Test). The degradation of Amphopropionates C12 -18 obtained 85.7 % after 28 days (measured by COD, criterion: >70%). Inhibition of the inoculum was observed in the TTC test at the concentration of 100 ml/L and higher.

According to producer information solid content is 39 - 41 %. The test substance manufactured before 1992 was produced out of ethyl acrylate and contained 6 % ethanol, pH (10 % at 20°C): 9-10

 

Overall, Amphopropionates C12 -18 is considered to be readily biodegradable and fulfilling the 10-d window criterion.