1-diethylaminopropan-2-ol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-diethylaminopropan-2-ol

EC Number:

224-537-7

EC Name:

1-diethylaminopropan-2-ol

Cas Number:

4402-32-8

Molecular formula:

C7H17NO

IUPAC Name:

1-(diethylamino)propan-2-ol

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solution was prepared by using a stock solution which was prepared by mixing the test sample and medium, and stirring them until being dissolved.
pH adjusted; Test solution was prepared by using a stock solution prepared by adjusting pH around medium.
- Controls: medium without test item

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchneriella suhcapitata
- Source: American Type Culture Collection; Strain number: ATCC 22662
- Method of cultivation:

Study design

Test type:

other: rotary shaking

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

21-24°C (±2°C)

pH:

pH of test solutions (pH unadjusted): 7.9 - 9.8
pH of test solutions (pH adjusted): start - 7.9
end - 8.7 - 10.0

Nominal and measured concentrations:

1, 3.16, 10, 31.6 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Sterilized 500 mL Erlenmeyer flask
- Type: closed
- Initial cells density: 0.5 x 104 cells/mL
- Volume of test solution: 600 mI/control level (100 mI/test vessel); 300 mI/exposure level (100 mI/test vessel)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: with and without
- Light intensity and quality: Nominal 90 µmoI·m-2·s-1 ± 20% (within±15% from the average light intensity). Continuous illumination provided by fluorescent lights with wavelength range of 400-700nm.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Remarks:

pH adjusted

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Remarks:

pH unadjusted

Effect conc.:

18.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Remarks:

pH adjusted

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Remarks:

pH unadjusted

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

ErC50 of the test item based on the growth rate was > 100 mg/L.
NOEC based on growth rate was determined to be >= 100 mg/L.