3,4-dicyanoaniline

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer revewed publication

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity of Aminophenyl and Nitrophenyl Ethers, Sulfides, And Disulfides

Author:

Edmond Lavoie, Lorraine Tulley, Elizabeth Fow and Dietrich Hoffmann

Year:

1979

Bibliographic source:

Mutation Research, 67 (1979) 123-131

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

The test chemical 4-Aminophenyl sulfone was studied to for its ability to induce mutations in strains of Salmonella typhimurium.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-Diaminodiphenyl Sulphone

Cas Number:

80-08-0

Molecular formula:

C12H12N2O2S

IUPAC Name:

4,4'-Diaminodiphenyl Sulphone

Details on test material:

- Name of test material: 4-Aminophenyl sulfone
- Molecular formula: C12H12N2O2S
- Molecular weight: 248.305 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Specific details on test material used for the study:

- Name of test material: 4-Aminophenyl sulfone
- Molecular formula: C12H12N2O2S
- Molecular weight: 248.305 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA98 and TA100

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

with and without

Metabolic activation system:

The S9 fraction was prepared Male Fisher-344 rats from Charles River injected with Aroclor 1254

Test concentrations with justification for top dose:

0, 25, 50, 100, 250 or 500 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The chemical was soluble in DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar

DURATION
- Preincubation period: No data
- Exposure duration: No data
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: Duplicate

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data

OTHER: No data

Rationale for test conditions:

No data

Evaluation criteria:

Compounds were considered mutagenic when the average number of histidine revertant colonies (His+ rev.) obtained at any dose level assayed were at least twice that of the solvent control

Statistics:

No data

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA98 and TA100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data

Any other information on results incl. tables

Table: Results from the mutagenic study for 4-Aminophenyl sulfone

Dose	His revertants			TA100/-S9/ % survival	TA100/+S9/ % survival
	TA100/+S9	TA100/-S9	TA98/+S9
500	163	153	98	39	112
250	171	152	108	80	98
100	174	163	94	97	107
50	139	134	113	102	89
25	141	146	113	105	90
DMSO (50µL)	135±16	160±12	78±110	100	100

Applicant's summary and conclusion

Conclusions:

4-Aminophenyl sulfone(80-08-0) is not mutagenic to the Salmonella typhimurium TA100 and TA98 in the presence and absence of S9 metabolic activation system.

Executive summary:

4-Aminophenyl sulfone was studied for its ability to induce mutations in strains of Salmonella typhimurium. The test compound was dissolved in DMSO and was tested at concentration of 0, 25, 50, 100, 250 or 500 µg/plate using Salmonella typhimurium TA100 and TA98 in the presence and absence of S9 metabolic activation system. 4-Aminophenyl sulfone is not mutagenic to the Salmonella typhimurium TA100 and TA98 in the presence and absence of S9 metabolic activation system.